Detection of uncommon G3P[3] rotavirus A (RVA) strain in rat possessing a human RVA-like VP6 and a novel NSP2 genotype

Giovanni Ianiro, Ilaria Di Bartolo, Luca De Sabato, Guglielmo Pampiglione, Franco M. Ruggeri, Fabio Ostanello

Research output: Contribution to journalArticle

4 Citations (Scopus)

Abstract

Rotavirus is one of the leading causes of acute gastroenteritis in infants and young children. RVAs infect not only humans but also a wide range of mammals including rats, which represent a reservoir of several other zoonotic pathogens. Due to the segmented nature of the RVA genome, animal RVA strains can easily adapt to the human host by reassortment with co-infecting human viruses. This study aims to detect and characterize RVA in the intestinal content of Italian sinantropic rats (Rattus rattus). Out of 40 samples examined following molecular approach, one resulted positive for RVA. The molecular characterization of VP1-4, 6 and 7, and NSP1-5 genes by sequencing revealed the genomic constellation G3-P[3]-I1-R11-C11-M10-A22-N18-T14-E18-H13. This uncommon genomic combination includes: the VP1-4,VP7, the NSP1, 3, 4 and 5 gene segments, closely related to those of RVA from rodents, the N18 novel genotype established for the NSP2 gene segment and the human Wa-like VP6 gene, suggesting interspecies reassortment.

Original languageEnglish
Pages (from-to)206-211
Number of pages6
JournalInfection, Genetics and Evolution
Volume53
DOIs
Publication statusPublished - Sep 1 2017

Fingerprint

Rotavirus A
Rotavirus
genotype
Genotype
gene
rats
genomics
gastroenteritis
genes
Genes
vertebrate viruses
Rattus rattus
rodent
virus
Gastrointestinal Contents
mammal
genome
pathogen
Zoonoses
Gastroenteritis

Keywords

  • G3P[3]
  • Group A rotavirus
  • Italy
  • NSP2
  • Rat
  • Reassortment

ASJC Scopus subject areas

  • Microbiology
  • Ecology, Evolution, Behavior and Systematics
  • Molecular Biology
  • Genetics
  • Microbiology (medical)
  • Infectious Diseases

Cite this

Detection of uncommon G3P[3] rotavirus A (RVA) strain in rat possessing a human RVA-like VP6 and a novel NSP2 genotype. / Ianiro, Giovanni; Di Bartolo, Ilaria; De Sabato, Luca; Pampiglione, Guglielmo; Ruggeri, Franco M.; Ostanello, Fabio.

In: Infection, Genetics and Evolution, Vol. 53, 01.09.2017, p. 206-211.

Research output: Contribution to journalArticle

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