Detecção de genótipos de Giardia duodenalis zoonóticos e específicos de ruminantes domésticos em lagartos selvagens

Translated title of the contribution: Detection of zoonotic and livestock-specific assemblages of Giardia duodenalis in free-living wild lizards

Aurora Reboredo-Fernández, Elvira Ares-Mazás, Pedro Galán, Simone Mario Cacciò, Hipólito Gómez-Couso

Research output: Contribution to journalArticle

Abstract

Giardia duodenalis is a zoonotic parasite that infects the gut of a wide range of vertebrates, including numerous wildlife species. However, little is known about this protozoan parasite in reptiles. Fecal samples from 31 wild lizards were collected in Galicia (northwest Spain) and screened for the presence of Giardia by PCR amplification and sequencing of the ITS1-5.8S-ITS2 region in the ribosomal unit. This allowed detection of the parasite in 5 samples (16.1%), and enabled identification of G. duodenalis assemblage A2 in two samples of Iberian rock lizard (Iberolacerta monticola), G. duodenalis assemblage B in other two samples of I. monticola, and G. duodenalis assemblage E in one sample of Bocage’s wall lizard (Podarcis bocagei). The results obtained after PCR amplification and sequencing of the SSU-rDNA gene confirmed the presence of G. duodenalis assemblage A in two samples of I. monticola. This is the first report of G. duodenalis in free-living lizards, although further studies are needed to distinguish between actual infection and mechanical dissemination of cysts. The detection of zoonotic and livestock-specific assemblages of G. duodenalis demonstrates the wide environmental contamination by this parasite, possibly due to human activities.

Original languagePortuguese
Pages (from-to)395-399
Number of pages5
JournalRevista Brasileira de Parasitologia Veterinaria
Volume26
Issue number3
DOIs
Publication statusPublished - Jul 1 2017

Fingerprint

Giardia lamblia
Lizards
Zoonoses
Livestock
lizards
livestock
Parasites
parasites
sampling
Giardia
Polymerase Chain Reaction
Reptiles
Ribosomal DNA
varespladib methyl
Human Activities
Spain
reptiles
anthropogenic activities
Protozoa
Vertebrates

Keywords

  • Giardia duodenalis
  • Wild reptiles
  • Zoonotic assemblages

ASJC Scopus subject areas

  • Parasitology
  • veterinary(all)

Cite this

Detecção de genótipos de Giardia duodenalis zoonóticos e específicos de ruminantes domésticos em lagartos selvagens. / Reboredo-Fernández, Aurora; Ares-Mazás, Elvira; Galán, Pedro; Cacciò, Simone Mario; Gómez-Couso, Hipólito.

In: Revista Brasileira de Parasitologia Veterinaria, Vol. 26, No. 3, 01.07.2017, p. 395-399.

Research output: Contribution to journalArticle

Reboredo-Fernández, Aurora ; Ares-Mazás, Elvira ; Galán, Pedro ; Cacciò, Simone Mario ; Gómez-Couso, Hipólito. / Detecção de genótipos de Giardia duodenalis zoonóticos e específicos de ruminantes domésticos em lagartos selvagens. In: Revista Brasileira de Parasitologia Veterinaria. 2017 ; Vol. 26, No. 3. pp. 395-399.
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