Determinants of platelet activation in hypertensives with microalbuminuria

Maria Teresa Guagnano, Patrizia Ferroni, Francesca Santilli, Vincenzo Paoletti, Maria Rosaria Manigrasso, Lea Pescara, Chiara Cuccurullo, Giovanni Ciabattoni, Giovanni Davì

Research output: Contribution to journalArticle

Abstract

Microalbuminuria is a predictor of adverse outcome in hypertension. We evaluated in vivo platelet activation, by urinary 11-dehydrothromboxane (TX)B2 and plasma P-selectin, in hypertensives with or without microalbuminuria, and its possible association with oxidative stress, by urinary 8-iso-prostaglandin (PG)F and endothelial dysfunction. Sixty essential hypertensive patients, with (n = 30) or without (n = 30) microalbuminuria, and 30 controls were studied. Endothelial function was assessed by nitric oxide products, intercellular adhesion molecule (ICAM)-1, and asymmetric dimethylarginine (ADMA) levels. Urinary 11-dehydro-TXB2 excretion was higher in microalbuminuric (median 805 pg/mg creatinine) compared to nonmicroalbuminuric patients or controls (414 and 291 pg/mg, respectively; P <0.0001). Plasma P-selectin was significantly higher in patients with microalbuminuria (median 136 ng/ml) as compared to those without microalbuminuria or controls (85 and 65 ng/ml; P <0.0001). Urinary 8-iso-PGF excretion was also enhanced in microalbuminuric (median 279 pg/mg creatinine) compared to nonmicroalbuminuric patients or controls (157 and 146 pg/mg, respectively; P <0.0001). A significant impairment in endothelial function was found in microalbuminuric patients, with decreased nitric oxide and increased ICAM-1 and ADMA levels. Multivariate regression analysis showed that urinary 8-iso-PGF excretion (beta = 0.49; P <0.0001) and microalbuminuria (beta = 0.36; P <0.001) were independently related to 11-dehydro-TXB2 in hypertensives. Vitamin E supplementation (900 mg daily for 1 month) in 10 hypertensives with microalbuminuria was associated with normalization in median 11-dehydro-TXB2 and 8-iso-PGF. We conclude that lipid peroxidation is a major determinant of persistent platelet activation in hypertensive patients with microalbuminuria.

Original languageEnglish
Pages (from-to)922-927
Number of pages6
JournalFree Radical Biology and Medicine
Volume46
Issue number7
DOIs
Publication statusPublished - Apr 1 2009

Keywords

  • Hypertension
  • Lipid peroxidation
  • Microalbuminuria
  • Platelet activation

ASJC Scopus subject areas

  • Biochemistry
  • Physiology (medical)

Fingerprint Dive into the research topics of 'Determinants of platelet activation in hypertensives with microalbuminuria'. Together they form a unique fingerprint.

  • Cite this

    Guagnano, M. T., Ferroni, P., Santilli, F., Paoletti, V., Manigrasso, M. R., Pescara, L., Cuccurullo, C., Ciabattoni, G., & Davì, G. (2009). Determinants of platelet activation in hypertensives with microalbuminuria. Free Radical Biology and Medicine, 46(7), 922-927. https://doi.org/10.1016/j.freeradbiomed.2009.01.005