Abstract
Following preliminary in-vitro experiments, platelet-leukocyte conjugates and their determinants were evaluated in citrated whole blood from 349 subjects (209 women, age 16-92 years) randomly recruited from the general population. Platelet activation by ADP/collagen but not leukocyte stimulation by fMLP or LTB4 resulted in formation of platelet conjugates with PMN or monocytes. In the population study, mixed cell conjugates, platelet P-selectin and leukocyte CD I I b were measured by flow cytometry both at baseline and after in-vitro stimulation with ADP/ collagen. The latter significantly increased platelet conjugates with either PMN or monocytes, platelet P-selectin and leukocyte CID I I b expression. Platelet count significantly correlated with platelet-PMN, platelet-monocyte conjugates and P-selectin both at baseline and upon stimulation. In all conditions, both conjugate levels correlated with each other, when adjusted for gender, age and platelet count. Age correlated with platelet-PMN conjugate numbers in basal and stimulated conditions and with basal P-selectin. ADP/collagen stimulation resulted in higher P-selectin and conjugates values in women. Among risk factors, a significant correlation was found between conjugate and glucose levels. In conclusion, the presence and formation in whole blood from a large population of platelet-leukocyte conjugates reflects primary platelet - but not leukocyte - activation and varies with gender, age, platelet count and blood glucose.
| Original language | English |
|---|---|
| Pages (from-to) | 1276-1284 |
| Number of pages | 9 |
| Journal | Thrombosis and Haemostasis |
| Volume | 98 |
| Issue number | 6 |
| DOIs | |
| Publication status | Published - Dec 2007 |
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Keywords
- Cell activation markers
- Flow cytometry
- General population
- Platelet P-selectin
- Platelet-leukocyte conjugates
ASJC Scopus subject areas
- Hematology
Cite this
Determinants of platelet conjugate formation with polymorphonuclear leukocytes or monocytes in whole blood. / Izzi, Benedetta; Pampuch, Agnieszka; Costanzo, Simona; Vohnout, Branislav; Iacoviello, Licia; Cerletti, Chiara; de Gaetano, Giovanni.
In: Thrombosis and Haemostasis, Vol. 98, No. 6, 12.2007, p. 1276-1284.Research output: Contribution to journal › Article
}
TY - JOUR
T1 - Determinants of platelet conjugate formation with polymorphonuclear leukocytes or monocytes in whole blood
AU - Izzi, Benedetta
AU - Pampuch, Agnieszka
AU - Costanzo, Simona
AU - Vohnout, Branislav
AU - Iacoviello, Licia
AU - Cerletti, Chiara
AU - de Gaetano, Giovanni
PY - 2007/12
Y1 - 2007/12
N2 - Following preliminary in-vitro experiments, platelet-leukocyte conjugates and their determinants were evaluated in citrated whole blood from 349 subjects (209 women, age 16-92 years) randomly recruited from the general population. Platelet activation by ADP/collagen but not leukocyte stimulation by fMLP or LTB4 resulted in formation of platelet conjugates with PMN or monocytes. In the population study, mixed cell conjugates, platelet P-selectin and leukocyte CD I I b were measured by flow cytometry both at baseline and after in-vitro stimulation with ADP/ collagen. The latter significantly increased platelet conjugates with either PMN or monocytes, platelet P-selectin and leukocyte CID I I b expression. Platelet count significantly correlated with platelet-PMN, platelet-monocyte conjugates and P-selectin both at baseline and upon stimulation. In all conditions, both conjugate levels correlated with each other, when adjusted for gender, age and platelet count. Age correlated with platelet-PMN conjugate numbers in basal and stimulated conditions and with basal P-selectin. ADP/collagen stimulation resulted in higher P-selectin and conjugates values in women. Among risk factors, a significant correlation was found between conjugate and glucose levels. In conclusion, the presence and formation in whole blood from a large population of platelet-leukocyte conjugates reflects primary platelet - but not leukocyte - activation and varies with gender, age, platelet count and blood glucose.
AB - Following preliminary in-vitro experiments, platelet-leukocyte conjugates and their determinants were evaluated in citrated whole blood from 349 subjects (209 women, age 16-92 years) randomly recruited from the general population. Platelet activation by ADP/collagen but not leukocyte stimulation by fMLP or LTB4 resulted in formation of platelet conjugates with PMN or monocytes. In the population study, mixed cell conjugates, platelet P-selectin and leukocyte CD I I b were measured by flow cytometry both at baseline and after in-vitro stimulation with ADP/ collagen. The latter significantly increased platelet conjugates with either PMN or monocytes, platelet P-selectin and leukocyte CID I I b expression. Platelet count significantly correlated with platelet-PMN, platelet-monocyte conjugates and P-selectin both at baseline and upon stimulation. In all conditions, both conjugate levels correlated with each other, when adjusted for gender, age and platelet count. Age correlated with platelet-PMN conjugate numbers in basal and stimulated conditions and with basal P-selectin. ADP/collagen stimulation resulted in higher P-selectin and conjugates values in women. Among risk factors, a significant correlation was found between conjugate and glucose levels. In conclusion, the presence and formation in whole blood from a large population of platelet-leukocyte conjugates reflects primary platelet - but not leukocyte - activation and varies with gender, age, platelet count and blood glucose.
KW - Cell activation markers
KW - Flow cytometry
KW - General population
KW - Platelet P-selectin
KW - Platelet-leukocyte conjugates
UR - http://www.scopus.com/inward/record.url?scp=36949004999&partnerID=8YFLogxK
UR - http://www.scopus.com/inward/citedby.url?scp=36949004999&partnerID=8YFLogxK
U2 - 10.1160/TH07-06-0383
DO - 10.1160/TH07-06-0383
M3 - Article
C2 - 18064325
AN - SCOPUS:36949004999
VL - 98
SP - 1276
EP - 1284
JO - Thrombosis and Haemostasis
JF - Thrombosis and Haemostasis
SN - 0340-6245
IS - 6
ER -