Determination of 2-arachidonoylglycerol by μSPE-LC-MS/MS

Natalia Battista, Manuel Sergi

Research output: Chapter in Book/Report/Conference proceedingChapter

Abstract

LC-MS/MS is a powerful analytical technique that provides unequivocal identification and reliable quantification of the analytes, using Selected Reaction Monitoring or Multi Reaction Monitoring acquisition mode. 2-Arachidonoylglycerol (2-AG) is the most abundant endocannabinoid, which plays a major role in a wide variety of physiological and pathological processes. Analysis of 2-AG by means of LC-MS/MS allows the detection of very low concentrations in biological samples. Here, we describe how to determine 2-AG levels in tiny samples of tissues and plasma through LC-MS/MS, by using very quick and easy to perform extraction procedures, with reduced solvent consumption.

Original languageEnglish
Title of host publicationMethods in Molecular Biology
PublisherHumana Press Inc.
Pages19-26
Number of pages8
Volume1412
DOIs
Publication statusPublished - 2016
Externally publishedYes

Publication series

NameMethods in Molecular Biology
Volume1412
ISSN (Print)10643745

Fingerprint

Physiological Phenomena
Endocannabinoids
Pathologic Processes
2-arachidonylglycerol

Keywords

  • 2-AG
  • LC-MS/MS
  • Plasma
  • Tissue
  • μSPE

ASJC Scopus subject areas

  • Molecular Biology
  • Genetics

Cite this

Battista, N., & Sergi, M. (2016). Determination of 2-arachidonoylglycerol by μSPE-LC-MS/MS. In Methods in Molecular Biology (Vol. 1412, pp. 19-26). (Methods in Molecular Biology; Vol. 1412). Humana Press Inc.. https://doi.org/10.1007/978-1-4939-3539-0_3

Determination of 2-arachidonoylglycerol by μSPE-LC-MS/MS. / Battista, Natalia; Sergi, Manuel.

Methods in Molecular Biology. Vol. 1412 Humana Press Inc., 2016. p. 19-26 (Methods in Molecular Biology; Vol. 1412).

Research output: Chapter in Book/Report/Conference proceedingChapter

Battista, N & Sergi, M 2016, Determination of 2-arachidonoylglycerol by μSPE-LC-MS/MS. in Methods in Molecular Biology. vol. 1412, Methods in Molecular Biology, vol. 1412, Humana Press Inc., pp. 19-26. https://doi.org/10.1007/978-1-4939-3539-0_3
Battista N, Sergi M. Determination of 2-arachidonoylglycerol by μSPE-LC-MS/MS. In Methods in Molecular Biology. Vol. 1412. Humana Press Inc. 2016. p. 19-26. (Methods in Molecular Biology). https://doi.org/10.1007/978-1-4939-3539-0_3
Battista, Natalia ; Sergi, Manuel. / Determination of 2-arachidonoylglycerol by μSPE-LC-MS/MS. Methods in Molecular Biology. Vol. 1412 Humana Press Inc., 2016. pp. 19-26 (Methods in Molecular Biology).
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