Abstract
LC-MS/MS is a powerful analytical technique that provides unequivocal identification and reliable quantification of the analytes, using Selected Reaction Monitoring or Multi Reaction Monitoring acquisition mode. 2-Arachidonoylglycerol (2-AG) is the most abundant endocannabinoid, which plays a major role in a wide variety of physiological and pathological processes. Analysis of 2-AG by means of LC-MS/MS allows the detection of very low concentrations in biological samples. Here, we describe how to determine 2-AG levels in tiny samples of tissues and plasma through LC-MS/MS, by using very quick and easy to perform extraction procedures, with reduced solvent consumption.
| Original language | English |
|---|---|
| Title of host publication | Methods in Molecular Biology |
| Publisher | Humana Press Inc. |
| Pages | 19-26 |
| Number of pages | 8 |
| Volume | 1412 |
| DOIs | |
| Publication status | Published - 2016 |
| Externally published | Yes |
Publication series
| Name | Methods in Molecular Biology |
|---|---|
| Volume | 1412 |
| ISSN (Print) | 10643745 |
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Keywords
- 2-AG
- LC-MS/MS
- Plasma
- Tissue
- μSPE
ASJC Scopus subject areas
- Molecular Biology
- Genetics
Cite this
Determination of 2-arachidonoylglycerol by μSPE-LC-MS/MS. / Battista, Natalia; Sergi, Manuel.
Methods in Molecular Biology. Vol. 1412 Humana Press Inc., 2016. p. 19-26 (Methods in Molecular Biology; Vol. 1412).Research output: Chapter in Book/Report/Conference proceeding › Chapter
}
TY - CHAP
T1 - Determination of 2-arachidonoylglycerol by μSPE-LC-MS/MS
AU - Battista, Natalia
AU - Sergi, Manuel
PY - 2016
Y1 - 2016
N2 - LC-MS/MS is a powerful analytical technique that provides unequivocal identification and reliable quantification of the analytes, using Selected Reaction Monitoring or Multi Reaction Monitoring acquisition mode. 2-Arachidonoylglycerol (2-AG) is the most abundant endocannabinoid, which plays a major role in a wide variety of physiological and pathological processes. Analysis of 2-AG by means of LC-MS/MS allows the detection of very low concentrations in biological samples. Here, we describe how to determine 2-AG levels in tiny samples of tissues and plasma through LC-MS/MS, by using very quick and easy to perform extraction procedures, with reduced solvent consumption.
AB - LC-MS/MS is a powerful analytical technique that provides unequivocal identification and reliable quantification of the analytes, using Selected Reaction Monitoring or Multi Reaction Monitoring acquisition mode. 2-Arachidonoylglycerol (2-AG) is the most abundant endocannabinoid, which plays a major role in a wide variety of physiological and pathological processes. Analysis of 2-AG by means of LC-MS/MS allows the detection of very low concentrations in biological samples. Here, we describe how to determine 2-AG levels in tiny samples of tissues and plasma through LC-MS/MS, by using very quick and easy to perform extraction procedures, with reduced solvent consumption.
KW - 2-AG
KW - LC-MS/MS
KW - Plasma
KW - Tissue
KW - μSPE
UR - http://www.scopus.com/inward/record.url?scp=84990246379&partnerID=8YFLogxK
UR - http://www.scopus.com/inward/citedby.url?scp=84990246379&partnerID=8YFLogxK
U2 - 10.1007/978-1-4939-3539-0_3
DO - 10.1007/978-1-4939-3539-0_3
M3 - Chapter
VL - 1412
T3 - Methods in Molecular Biology
SP - 19
EP - 26
BT - Methods in Molecular Biology
PB - Humana Press Inc.
ER -