Determination of 2-arachidonoylglycerol by μSPE-LC-MS/MS

Natalia Battista, Manuel Sergi

Research output: Chapter in Book/Report/Conference proceedingChapter


LC-MS/MS is a powerful analytical technique that provides unequivocal identification and reliable quantification of the analytes, using Selected Reaction Monitoring or Multi Reaction Monitoring acquisition mode. 2-Arachidonoylglycerol (2-AG) is the most abundant endocannabinoid, which plays a major role in a wide variety of physiological and pathological processes. Analysis of 2-AG by means of LC-MS/MS allows the detection of very low concentrations in biological samples. Here, we describe how to determine 2-AG levels in tiny samples of tissues and plasma through LC-MS/MS, by using very quick and easy to perform extraction procedures, with reduced solvent consumption.

Original languageEnglish
Title of host publicationMethods in Molecular Biology
PublisherHumana Press Inc.
Number of pages8
Publication statusPublished - 2016
Externally publishedYes

Publication series

NameMethods in Molecular Biology
ISSN (Print)10643745


  • 2-AG
  • LC-MS/MS
  • Plasma
  • Tissue
  • μSPE

ASJC Scopus subject areas

  • Molecular Biology
  • Genetics


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