Determination of a glycosyl subunit of human serum albumin by concanavalin A-Sepharose

Giovanni Candiano, Gian Marco Ghiggeri, Gerolamo Delfino, Fosco Cavatorta, Carlo Queirolo

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A new procedure has been devised for the isolation of a glycosyl subunit of albumin from human serum. After the selective removal from serum proteins with affinity chromatography on Blue-Sepharose CL-6B, albumin was applied to a column of concanavalin-A Sepharose which resolved the protein in two subunits with different specific colour activity for carbohydrates, as tested with thiobarbituric acid assay. The glycosyl albumin bound to concanavalin-A Sepharose was homogeneous when examined by immunoelectrophoresis and sodium dodecyl-sulphate polyacrylamide electrophoresis, whereas it showed a microheterogeneity when tested by isoelectric focusing. The procedure was applied to a model system as well as to serum from normal and diabetic patients.

Original languageEnglish
Pages (from-to)29-40
Number of pages12
JournalClinica Chimica Acta
Issue number1
Publication statusPublished - Feb 28 1983

ASJC Scopus subject areas

  • Biochemistry
  • Clinical Biochemistry


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