Determination of a glycosyl subunit of human serum albumin by concanavalin A-Sepharose

Giovanni Candiano, Gian Marco Ghiggeri, Gerolamo Delfino, Fosco Cavatorta, Carlo Queirolo

Research output: Contribution to journalArticle

Abstract

A new procedure has been devised for the isolation of a glycosyl subunit of albumin from human serum. After the selective removal from serum proteins with affinity chromatography on Blue-Sepharose CL-6B, albumin was applied to a column of concanavalin-A Sepharose which resolved the protein in two subunits with different specific colour activity for carbohydrates, as tested with thiobarbituric acid assay. The glycosyl albumin bound to concanavalin-A Sepharose was homogeneous when examined by immunoelectrophoresis and sodium dodecyl-sulphate polyacrylamide electrophoresis, whereas it showed a microheterogeneity when tested by isoelectric focusing. The procedure was applied to a model system as well as to serum from normal and diabetic patients.

Original languageEnglish
Pages (from-to)29-40
Number of pages12
JournalClinica Chimica Acta
Volume128
Issue number1
DOIs
Publication statusPublished - Feb 28 1983

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Serum Albumin
Albumins
Affinity chromatography
Immunoelectrophoresis
Isoelectric Focusing
Electrophoresis
Serum
Affinity Chromatography
Sodium Dodecyl Sulfate
Blood Proteins
Assays
Color
Carbohydrates
Proteins
concanavalin A-sepharose
glycosylated serum albumin
polyacrylamide
cibacron blue-sepharose
thiobarbituric acid

ASJC Scopus subject areas

  • Biochemistry
  • Clinical Biochemistry

Cite this

Determination of a glycosyl subunit of human serum albumin by concanavalin A-Sepharose. / Candiano, Giovanni; Ghiggeri, Gian Marco; Delfino, Gerolamo; Cavatorta, Fosco; Queirolo, Carlo.

In: Clinica Chimica Acta, Vol. 128, No. 1, 28.02.1983, p. 29-40.

Research output: Contribution to journalArticle

Candiano, Giovanni ; Ghiggeri, Gian Marco ; Delfino, Gerolamo ; Cavatorta, Fosco ; Queirolo, Carlo. / Determination of a glycosyl subunit of human serum albumin by concanavalin A-Sepharose. In: Clinica Chimica Acta. 1983 ; Vol. 128, No. 1. pp. 29-40.
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