Determination of abacavir, amprenavir, didanosine, efavirenz, nevirapine, and stavudine concentration in human plasma by MALDI-TOF/TOF

Stefania Notari, Carmine Mancone, Tonino Alonzi, Marco Tripodi, Pasquale Narciso, Paolo Ascenzi

Research output: Contribution to journalArticle

Abstract

The interest in therapeutic drug monitoring (TDM) of antiretroviral drugs has grown significantly since highly active antiretroviral therapy (HAART) became a standard of care in clinical practice. TDM is useful to determine the best dosage regimen adapted to each patient. Here, we apply MALDI-TOF/TOF technology to quantify abacavir, amprenavir, didanosine, efavirenz, nevirapine, and stavudine in the plasma of HIV-infected patients, by standard additions analysis. Regression of standard additions was linear over the whole anti-HIV concentration range explored (1.00 × 10-2-1.00 pmol/μL). The absolute recovery ranged between 80% and 110%. Values of the drug concentration determined by MALDI-TOF/TOF were in the range of 1.00 × 10-2-1.00 pmol/μL. The limit of quantification value was 1.00 × 10-2 pmol/μL for abacavir, amprenavir, didanosine, efavirenz, nevirapine, and stavudine.

Original languageEnglish
Pages (from-to)249-257
Number of pages9
JournalJournal of Chromatography B: Analytical Technologies in the Biomedical and Life Sciences
Volume863
Issue number2
DOIs
Publication statusPublished - Mar 1 2008

Fingerprint

efavirenz
Stavudine
Didanosine
Plasma (human)
Nevirapine
Drug Monitoring
Matrix-Assisted Laser Desorption-Ionization Mass Spectrometry
HIV
Highly Active Antiretroviral Therapy
Standard of Care
Pharmaceutical Preparations
Technology
Monitoring
Plasmas
Recovery
abacavir
amprenavir

Keywords

  • Anti-HIV drug determination
  • Human plasma
  • MALDI-TOF/TOF

ASJC Scopus subject areas

  • Biochemistry

Cite this

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title = "Determination of abacavir, amprenavir, didanosine, efavirenz, nevirapine, and stavudine concentration in human plasma by MALDI-TOF/TOF",
abstract = "The interest in therapeutic drug monitoring (TDM) of antiretroviral drugs has grown significantly since highly active antiretroviral therapy (HAART) became a standard of care in clinical practice. TDM is useful to determine the best dosage regimen adapted to each patient. Here, we apply MALDI-TOF/TOF technology to quantify abacavir, amprenavir, didanosine, efavirenz, nevirapine, and stavudine in the plasma of HIV-infected patients, by standard additions analysis. Regression of standard additions was linear over the whole anti-HIV concentration range explored (1.00 × 10-2-1.00 pmol/μL). The absolute recovery ranged between 80{\%} and 110{\%}. Values of the drug concentration determined by MALDI-TOF/TOF were in the range of 1.00 × 10-2-1.00 pmol/μL. The limit of quantification value was 1.00 × 10-2 pmol/μL for abacavir, amprenavir, didanosine, efavirenz, nevirapine, and stavudine.",
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AU - Notari, Stefania

AU - Mancone, Carmine

AU - Alonzi, Tonino

AU - Tripodi, Marco

AU - Narciso, Pasquale

AU - Ascenzi, Paolo

PY - 2008/3/1

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N2 - The interest in therapeutic drug monitoring (TDM) of antiretroviral drugs has grown significantly since highly active antiretroviral therapy (HAART) became a standard of care in clinical practice. TDM is useful to determine the best dosage regimen adapted to each patient. Here, we apply MALDI-TOF/TOF technology to quantify abacavir, amprenavir, didanosine, efavirenz, nevirapine, and stavudine in the plasma of HIV-infected patients, by standard additions analysis. Regression of standard additions was linear over the whole anti-HIV concentration range explored (1.00 × 10-2-1.00 pmol/μL). The absolute recovery ranged between 80% and 110%. Values of the drug concentration determined by MALDI-TOF/TOF were in the range of 1.00 × 10-2-1.00 pmol/μL. The limit of quantification value was 1.00 × 10-2 pmol/μL for abacavir, amprenavir, didanosine, efavirenz, nevirapine, and stavudine.

AB - The interest in therapeutic drug monitoring (TDM) of antiretroviral drugs has grown significantly since highly active antiretroviral therapy (HAART) became a standard of care in clinical practice. TDM is useful to determine the best dosage regimen adapted to each patient. Here, we apply MALDI-TOF/TOF technology to quantify abacavir, amprenavir, didanosine, efavirenz, nevirapine, and stavudine in the plasma of HIV-infected patients, by standard additions analysis. Regression of standard additions was linear over the whole anti-HIV concentration range explored (1.00 × 10-2-1.00 pmol/μL). The absolute recovery ranged between 80% and 110%. Values of the drug concentration determined by MALDI-TOF/TOF were in the range of 1.00 × 10-2-1.00 pmol/μL. The limit of quantification value was 1.00 × 10-2 pmol/μL for abacavir, amprenavir, didanosine, efavirenz, nevirapine, and stavudine.

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