The interest in therapeutic drug monitoring (TDM) of antiretroviral drugs has grown significantly since highly active antiretroviral therapy (HAART) became a standard of care in clinical practice. TDM is useful to determine the best dosage regimen adapted to each patient. Here, we apply MALDI-TOF/TOF technology to quantify abacavir, amprenavir, didanosine, efavirenz, nevirapine, and stavudine in the plasma of HIV-infected patients, by standard additions analysis. Regression of standard additions was linear over the whole anti-HIV concentration range explored (1.00 × 10-2-1.00 pmol/μL). The absolute recovery ranged between 80% and 110%. Values of the drug concentration determined by MALDI-TOF/TOF were in the range of 1.00 × 10-2-1.00 pmol/μL. The limit of quantification value was 1.00 × 10-2 pmol/μL for abacavir, amprenavir, didanosine, efavirenz, nevirapine, and stavudine.
|Number of pages||9|
|Journal||Journal of Chromatography B: Analytical Technologies in the Biomedical and Life Sciences|
|Publication status||Published - Mar 1 2008|
- Anti-HIV drug determination
- Human plasma
ASJC Scopus subject areas