DETERMINAZIONE DEGLI ANTICORPI ANTI CITOMEGALOVIRUS MEDIANTE LA METODICA IMMUNOPEROSSIDASICA: CONFRONTO CON LE METODICHE DI FISSAZIONE DEL COMPLEMENTO E DI IMMUNOFLUORESCENZA

Translated title of the contribution: Determination of anti cytomegalovirus antibodies by the immunoperoxidase technique: comparison with complement fixation and immunofluorescence techniques

G. Gerna, C. J. McCloud, E. Cattaneo, P. Cereda, M. Gallina

Research output: Contribution to journalArticle

Abstract

Indirect immunoperoxidase technique (IIP) has been employed for determination of IgG antibody titer to cytomegalovirus (CMV) in sera from 110 blood donors. Titers have been compared with complement fixation (CF) and indirect fluorescent antibody (IFA) technique titers, the latter being applied to determination of all specific antibody classes. Within the variability range of the quality of reagents employed in different tests, it seems reasonable to conclude that the IIP technique is as sensitive as IFA and much more sensitive than the CF test. Considering that the CF test is able to detect only IgG antibody, the advantage of using immunohistochemical techniques is obvious. Moreover IIP does not need the fluorescence microscope and reading the results is easier and can be delayed. Non specific staining of the Golgi area is shared by both techniques, but endpoint determination has to take into account only the nuclear virus specific labelling of the inclusions.

Original languageItalian
Pages (from-to)126-135
Number of pages10
JournalBollettino dell'Istituto Sieroterapico Milanese
Volume54
Issue number2
Publication statusPublished - 1975

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Cytomegalovirus
Immunoenzyme Techniques
Fluorescent Antibody Technique
Complement Fixation Tests
Anti-Idiotypic Antibodies
Antibodies
Immunoglobulin G
Endpoint Determination
Immunoglobulin Isotypes
Indirect Fluorescent Antibody Technique
Blood Donors
Reading
Fluorescence
Staining and Labeling
Viruses
Serum

ASJC Scopus subject areas

  • Immunology

Cite this

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title = "DETERMINAZIONE DEGLI ANTICORPI ANTI CITOMEGALOVIRUS MEDIANTE LA METODICA IMMUNOPEROSSIDASICA: CONFRONTO CON LE METODICHE DI FISSAZIONE DEL COMPLEMENTO E DI IMMUNOFLUORESCENZA",
abstract = "Indirect immunoperoxidase technique (IIP) has been employed for determination of IgG antibody titer to cytomegalovirus (CMV) in sera from 110 blood donors. Titers have been compared with complement fixation (CF) and indirect fluorescent antibody (IFA) technique titers, the latter being applied to determination of all specific antibody classes. Within the variability range of the quality of reagents employed in different tests, it seems reasonable to conclude that the IIP technique is as sensitive as IFA and much more sensitive than the CF test. Considering that the CF test is able to detect only IgG antibody, the advantage of using immunohistochemical techniques is obvious. Moreover IIP does not need the fluorescence microscope and reading the results is easier and can be delayed. Non specific staining of the Golgi area is shared by both techniques, but endpoint determination has to take into account only the nuclear virus specific labelling of the inclusions.",
author = "G. Gerna and McCloud, {C. J.} and E. Cattaneo and P. Cereda and M. Gallina",
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AU - Gerna, G.

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