Determination of antibodies to extractable nuclear antigens by commercial kits

A multicenter study

P. Cordiali Fei, G. D'Agosto, F. Ameglio, G. Valesini, C. Alessandri, A. Farsi, M. P. Domenighetti, A. Passaleva, F. Scamardella, R. Neri, S. Bombardieri, M. Quinzanini, F. Franceschini, F. Chiarotti

Research output: Contribution to journalArticle

8 Citations (Scopus)

Abstract

Several enzyme immunoassays for serum antibodies to extractable nuclear antigen have recently become available. The aim of this study was to evaluate the results obtained with: (1) the same kit under different conditions; (2) different enzyme immunoassays; (3) Western blot and enzyme immunoassays. Twenty-five sera from patients with autoimmune disorders were tested in five different laboratories by one Western blot and four enzyme immunoassay commercial kits. The different methods produced comparable qualitative results. However, semiquantitative evaluation, based on a cut-off value (index), yielded different results due both to laboratory conditions and to the kits employed. Standardization of commercial products and methods should be improved so that the results of different laboratories can be compared and large-scale and follow-up studies conducted. Western blot analysis could also be useful to analyze complex reactivities, although greater experience is necessary to interpret these results correctly.

Original languageEnglish
Pages (from-to)29-33
Number of pages5
JournalInternational Journal of Clinical & Laboratory Research
Volume28
Issue number1
DOIs
Publication statusPublished - Apr 1998

Fingerprint

Nuclear Antigens
Immunoenzyme Techniques
Multicenter Studies
Antibodies
Western Blotting
Enzymes
Serum
Standardization

Keywords

  • Anti-extractable nuclear antigen antibody
  • Autoimmune diseases
  • Enzyme immunoassay
  • Western blot assay

ASJC Scopus subject areas

  • Clinical Biochemistry

Cite this

Determination of antibodies to extractable nuclear antigens by commercial kits : A multicenter study. / Cordiali Fei, P.; D'Agosto, G.; Ameglio, F.; Valesini, G.; Alessandri, C.; Farsi, A.; Domenighetti, M. P.; Passaleva, A.; Scamardella, F.; Neri, R.; Bombardieri, S.; Quinzanini, M.; Franceschini, F.; Chiarotti, F.

In: International Journal of Clinical & Laboratory Research, Vol. 28, No. 1, 04.1998, p. 29-33.

Research output: Contribution to journalArticle

Cordiali Fei, P, D'Agosto, G, Ameglio, F, Valesini, G, Alessandri, C, Farsi, A, Domenighetti, MP, Passaleva, A, Scamardella, F, Neri, R, Bombardieri, S, Quinzanini, M, Franceschini, F & Chiarotti, F 1998, 'Determination of antibodies to extractable nuclear antigens by commercial kits: A multicenter study', International Journal of Clinical & Laboratory Research, vol. 28, no. 1, pp. 29-33. https://doi.org/10.1007/s005990050014
Cordiali Fei, P. ; D'Agosto, G. ; Ameglio, F. ; Valesini, G. ; Alessandri, C. ; Farsi, A. ; Domenighetti, M. P. ; Passaleva, A. ; Scamardella, F. ; Neri, R. ; Bombardieri, S. ; Quinzanini, M. ; Franceschini, F. ; Chiarotti, F. / Determination of antibodies to extractable nuclear antigens by commercial kits : A multicenter study. In: International Journal of Clinical & Laboratory Research. 1998 ; Vol. 28, No. 1. pp. 29-33.
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