Determination of argininosuccinate lyase in human serum by ion-pair reversed phase liquid chromatography

S. Canepari, V. Carunchio, A. M. Girelli, A. Messina

Research output: Contribution to journalArticle

Abstract

A new assay for argininosuccinate lyase based on the separation of the enzymatic reaction mixture components by an ion-pair reversed phase mechanism is reported. The determination of enzyme activity is performed after direct injection by UV detection of the fumarate formed. The chromatographic analysis time is 8 min and a detection limit of 0.4 U/L is achieved. The HPLC method is highly accurate, sensitive and precise. The simple procedure makes this method suitable for the routine determination of ASAL activity in human serum samples.

Original languageEnglish
Pages (from-to)171-174
Number of pages4
JournalBiomedical Chromatography
Volume9
Issue number4
DOIs
Publication statusPublished - 1995

Fingerprint

Argininosuccinate Lyase
Chromatographic analysis
Fumarates
Liquid chromatography
Direct injection
Enzyme activity
Reverse-Phase Chromatography
Assays
Ions
Serum
Human Activities
Limit of Detection
Chromatography
High Pressure Liquid Chromatography
Injections
Enzymes
allatostatin 1

ASJC Scopus subject areas

  • Biochemistry, Genetics and Molecular Biology(all)
  • Biochemistry
  • Clinical Biochemistry
  • Analytical Chemistry
  • Pharmacology

Cite this

Determination of argininosuccinate lyase in human serum by ion-pair reversed phase liquid chromatography. / Canepari, S.; Carunchio, V.; Girelli, A. M.; Messina, A.

In: Biomedical Chromatography, Vol. 9, No. 4, 1995, p. 171-174.

Research output: Contribution to journalArticle

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