Determination of aspartate aminotransferase activity by high-performance liquid chromatography

S. Canepari, V. Carunchio, A. M. Girelli, A. Messina

Research output: Contribution to journalArticlepeer-review

Abstract

A sensitive and reproducible assay of aspartate aminotransferase activity based on UV detection of the reaction products after their separation by HPLC is described. The main advantage is the direct measurement of the enzyme activity as micromoles of product (glutamate) formed within a known period of time without any coupled reaction. Further, with the chromatographic method, all components of the reaction mixture are identified, allowing the reaction course to be controlled and the possible presence of side-reactions to be monitored.

Original languageEnglish
Pages (from-to)191-195
Number of pages5
JournalJournal of Chromatography B: Biomedical Sciences and Applications
Volume656
Issue number1
DOIs
Publication statusPublished - Jun 3 1994

ASJC Scopus subject areas

  • Chemistry(all)

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