Determination of atherogenesis in apolipoprotein E-knockout mice

C. Napoli, W. Palinski, G. Di Minno, F. P. D'Armiento

Research output: Contribution to journalArticle

Abstract

Background and Aim: Genetically-modified mice are the preferred model for studies on atherogenesis. Conventional procedures for determining lesion size and composition may be not very accurate, especially when only very small lesions are present. We provide here a detailed characterization of lesion components and the computer-assisted quantitative determination of atherogenesis in apolipoprotein E-knockout (apoE-/-) mice. Methods and Results: Atherosclerotic lesions were detected at typical predilection sites in the aorta: the small curvature of the arch, the orifices of the brachiocephalic trunk, the left subclavia and common carotid artery, the branch sites of the mesenteric and renal arteries, the abdominal aorta, and the iliac bifurcation. We found that these lesions covered 20% of the aortic surface of apoE-/- on average compared with 1% of the wild type C57BL/6 mice (p-/- mice. Conclusions: Although some limitations may reduce the applicability of results obtained in mice to humans, the apoE-/- mouse appears to be suitable for investigation of the role of apolipoproteins, enzymes and receptors involved in lipid metabolism, lipid oxidation, vascular cells and their secretory products as well as the possible role of antioxidants and gene-therapy in reducing atherogenesis.

Original languageEnglish
Pages (from-to)209-215
Number of pages7
JournalNutrition, Metabolism and Cardiovascular Diseases
Volume10
Issue number4
Publication statusPublished - Aug 2000

Keywords

  • Apo-/- mice
  • Artery
  • Atherogenesis

ASJC Scopus subject areas

  • Food Science
  • Medicine (miscellaneous)
  • Cardiology and Cardiovascular Medicine
  • Endocrinology, Diabetes and Metabolism
  • Nutrition and Dietetics

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