Determination of brodimoprim and its hydroxy metabolite in human plasma, blood and urine by high-performance liquid chromatography

Flavio Gaspari, Luca Taiocchi, Maria Grazia Pochobradsky

Research output: Contribution to journalArticle

Abstract

A high-performance liquid chromatographic method was developed to measure the concentration of brodimoprim and its metabolite, hydroxybrodimoprim, in small volumes of blood, plasma and urine. The procedure involved a simple extraction step with chloroform, followed by chromatographic separation on a short reversed-phase column deactivated for the analysis of basic compounds. The column effluent was monitored by fluorescence (excitation wavelength 290 nm, emission wavelength 340 nm). The recoveries of both compounds were similar in all three biological fluids, and averaged 84 and 72%, respectively. The detection limit for both compounds reached 5 ng/ml. No endogenous compound interfered in the assay. The linearity of the method and its within- and between-day precision were analytically satisfactory.

Original languageEnglish
Pages (from-to)123-128
Number of pages6
JournalJournal of Chromatography B: Biomedical Sciences and Applications
Volume577
Issue number1
DOIs
Publication statusPublished - May 20 1992

ASJC Scopus subject areas

  • Chemistry(all)
  • Clinical Biochemistry
  • Molecular Medicine

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