Determination of creatinine in serum and urine by a rapid liquid-chromatographic method

R. Paroni, C. Arcelloni, I. Fermo, P. A. Bonini

Research output: Contribution to journalArticlepeer-review


We describe an HPLC ion-pair procedure for rapid and specific evaluation of creatinine in serum and urine. We used a 15 cm × 4.6 mm ODS column with a 50/50 (by vol) mixture of sodium decanesulfonic acid (10 mmol/L, pH 3.2) and methanol and measured absorbance at 236 nm. Serum (100 μL.) or 30-fold-diluted urine (100 μL) was added to 400 μL of acetone. After centrifugation, the supernates (300 μL) were dried, reconstituted with the mobile phase, and injected into the HPLC. Assay precision was tested for concentrations of 10, 29, and 130 mg/L and yielded, respectively, 3.1%, 2.1%, and 1.1% for within-day CV and 2.8%, 2.1%, and 2.2% for total CV. Analytical recovery was 102 (±6.7)%. Linearity was demonstrated in the 0-200 mg/L range for serum and 0-3.5 g/L range for urine (r ≥0.999). The detection limit for creatinine (signal-to-noise ratio = 3) was 0.5 mg/L. We used cimetidine for internal standardization. Correlation was good between this procedure and the Jaffé kinetic, the enzymatic (creatinine amidohydrolase), and the Fuller's earth alkaline picrate methods.

Original languageEnglish
Pages (from-to)830-836
Number of pages7
JournalClinical Chemistry
Issue number6
Publication statusPublished - 1990


  • Chromatography
  • Reversed-phase cimetidine as internal standard

ASJC Scopus subject areas

  • Clinical Biochemistry


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