Determination of ferric heme-human serum albumin by 1H NMR relaxometry

Mauro Fasano, Simona Baroni, Silvio Aime, Marco Mattu, Paolo Ascenzi

Research output: Contribution to journalArticle

3 Citations (Scopus)

Abstract

A new method for the accurate determination of ferric heme-human serum albumin (heme-HSA) at concentrations down to the physiological level, i.e., in the micromolar concentration range, is proposed. This method is based on the 1H NMR relaxometric properties of heme-HSA. Actually, the binding of the paramagnetic ferric heme to the primary binding site of HSA determines a strong paramagnetic enhancement of the water 1H NMR relaxation rate. Although a linear relationship may be seen by operating at 20 MHz on conventional electromagnets, the method here reported is improved by working at 0.02 MHz on a field-cycling instrument. This 1H NMR relaxometric method does not suffer from the presence in serum of heme catabolites (e.g., bilirubin) that affect significantly the optical determination of ferric heme-HSA in the micromolar concentration range. Paramagnetic ferric hemoglobin contribution may be selectively quenched by cyanide binding.

Original languageEnglish
Pages (from-to)64-67
Number of pages4
JournalJournal of Inorganic Biochemistry
Volume95
Issue number1
DOIs
Publication statusPublished - May 1 2003

Fingerprint

Heme
Serum Albumin
Nuclear magnetic resonance
Electromagnets
Magnets
Cyanides
Bilirubin
Hemoglobins
Binding Sites
Proton Magnetic Resonance Spectroscopy
Water
Serum

Keywords

  • H NMR relaxometry
  • Determination
  • Ferric heme-human serum albumin

ASJC Scopus subject areas

  • Biochemistry
  • Inorganic Chemistry

Cite this

Determination of ferric heme-human serum albumin by 1H NMR relaxometry. / Fasano, Mauro; Baroni, Simona; Aime, Silvio; Mattu, Marco; Ascenzi, Paolo.

In: Journal of Inorganic Biochemistry, Vol. 95, No. 1, 01.05.2003, p. 64-67.

Research output: Contribution to journalArticle

Fasano, Mauro ; Baroni, Simona ; Aime, Silvio ; Mattu, Marco ; Ascenzi, Paolo. / Determination of ferric heme-human serum albumin by 1H NMR relaxometry. In: Journal of Inorganic Biochemistry. 2003 ; Vol. 95, No. 1. pp. 64-67.
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