Abstract
A procedure was devised for the determination of the unconjugated non-protein-bound fraction of 3,4-dihydroxyphenylalanine (DOPA) and 3-O-methyl-DOPA (3-OMD) in plasma using a reversed-phase liquid chromatographic system coupled with electrochemical detection. Sample preparation involves rapid isolation of the unbound drugs from the drug-protein complex by ultrafiltration through a membrane with a molecular weight cut-off of 10 000 dalton. One chromatographic run requires less than 10 min. The relative standard deviation is <3% for the within-assay imprecision and <4% for the between-assay imprecision. The detection limits for DOPA and 3-OMD are 0.2 and 1.3 ng/ml, respectively.
| Original language | English |
|---|---|
| Pages (from-to) | 265-269 |
| Number of pages | 5 |
| Journal | Journal of Chromatography A |
| Volume | 553 |
| Issue number | C |
| DOIs | |
| Publication status | Published - Aug 16 1991 |
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ASJC Scopus subject areas
- Analytical Chemistry
- Clinical Biochemistry
- Molecular Medicine
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Determination of free DOPA and 3-O-methyl-DOPA in human plasma by high-performance liquid chromatography with electrochemical detection. / Melzi D'eril, G. V.; Rizzo, V.
In: Journal of Chromatography A, Vol. 553, No. C, 16.08.1991, p. 265-269.Research output: Contribution to journal › Article
}
TY - JOUR
T1 - Determination of free DOPA and 3-O-methyl-DOPA in human plasma by high-performance liquid chromatography with electrochemical detection
AU - Melzi D'eril, G. V.
AU - Rizzo, V.
PY - 1991/8/16
Y1 - 1991/8/16
N2 - A procedure was devised for the determination of the unconjugated non-protein-bound fraction of 3,4-dihydroxyphenylalanine (DOPA) and 3-O-methyl-DOPA (3-OMD) in plasma using a reversed-phase liquid chromatographic system coupled with electrochemical detection. Sample preparation involves rapid isolation of the unbound drugs from the drug-protein complex by ultrafiltration through a membrane with a molecular weight cut-off of 10 000 dalton. One chromatographic run requires less than 10 min. The relative standard deviation is <3% for the within-assay imprecision and <4% for the between-assay imprecision. The detection limits for DOPA and 3-OMD are 0.2 and 1.3 ng/ml, respectively.
AB - A procedure was devised for the determination of the unconjugated non-protein-bound fraction of 3,4-dihydroxyphenylalanine (DOPA) and 3-O-methyl-DOPA (3-OMD) in plasma using a reversed-phase liquid chromatographic system coupled with electrochemical detection. Sample preparation involves rapid isolation of the unbound drugs from the drug-protein complex by ultrafiltration through a membrane with a molecular weight cut-off of 10 000 dalton. One chromatographic run requires less than 10 min. The relative standard deviation is <3% for the within-assay imprecision and <4% for the between-assay imprecision. The detection limits for DOPA and 3-OMD are 0.2 and 1.3 ng/ml, respectively.
UR - http://www.scopus.com/inward/record.url?scp=0025766748&partnerID=8YFLogxK
UR - http://www.scopus.com/inward/citedby.url?scp=0025766748&partnerID=8YFLogxK
U2 - 10.1016/S0021-9673(01)88497-1
DO - 10.1016/S0021-9673(01)88497-1
M3 - Article
C2 - 1787158
AN - SCOPUS:0025766748
VL - 553
SP - 265
EP - 269
JO - Journal of Chromatography A
JF - Journal of Chromatography A
SN - 0021-9673
IS - C
ER -