TY - JOUR
T1 - Determination of free DOPA and 3-O-methyl-DOPA in human plasma by high-performance liquid chromatography with electrochemical detection
AU - Melzi D'eril, G. V.
AU - Rizzo, V.
PY - 1991/8/16
Y1 - 1991/8/16
N2 - A procedure was devised for the determination of the unconjugated non-protein-bound fraction of 3,4-dihydroxyphenylalanine (DOPA) and 3-O-methyl-DOPA (3-OMD) in plasma using a reversed-phase liquid chromatographic system coupled with electrochemical detection. Sample preparation involves rapid isolation of the unbound drugs from the drug-protein complex by ultrafiltration through a membrane with a molecular weight cut-off of 10 000 dalton. One chromatographic run requires less than 10 min. The relative standard deviation is <3% for the within-assay imprecision and <4% for the between-assay imprecision. The detection limits for DOPA and 3-OMD are 0.2 and 1.3 ng/ml, respectively.
AB - A procedure was devised for the determination of the unconjugated non-protein-bound fraction of 3,4-dihydroxyphenylalanine (DOPA) and 3-O-methyl-DOPA (3-OMD) in plasma using a reversed-phase liquid chromatographic system coupled with electrochemical detection. Sample preparation involves rapid isolation of the unbound drugs from the drug-protein complex by ultrafiltration through a membrane with a molecular weight cut-off of 10 000 dalton. One chromatographic run requires less than 10 min. The relative standard deviation is <3% for the within-assay imprecision and <4% for the between-assay imprecision. The detection limits for DOPA and 3-OMD are 0.2 and 1.3 ng/ml, respectively.
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U2 - 10.1016/S0021-9673(01)88497-1
DO - 10.1016/S0021-9673(01)88497-1
M3 - Article
C2 - 1787158
AN - SCOPUS:0025766748
VL - 553
SP - 265
EP - 269
JO - Journal of Chromatography A
JF - Journal of Chromatography A
SN - 0021-9673
IS - C
ER -