Determination of free DOPA and 3-O-methyl-DOPA in human plasma by high-performance liquid chromatography with electrochemical detection

G. V. Melzi D'eril, V. Rizzo

Research output: Contribution to journalArticlepeer-review

Abstract

A procedure was devised for the determination of the unconjugated non-protein-bound fraction of 3,4-dihydroxyphenylalanine (DOPA) and 3-O-methyl-DOPA (3-OMD) in plasma using a reversed-phase liquid chromatographic system coupled with electrochemical detection. Sample preparation involves rapid isolation of the unbound drugs from the drug-protein complex by ultrafiltration through a membrane with a molecular weight cut-off of 10 000 dalton. One chromatographic run requires less than 10 min. The relative standard deviation is <3% for the within-assay imprecision and <4% for the between-assay imprecision. The detection limits for DOPA and 3-OMD are 0.2 and 1.3 ng/ml, respectively.

Original languageEnglish
Pages (from-to)265-269
Number of pages5
JournalJournal of Chromatography A
Volume553
Issue numberC
DOIs
Publication statusPublished - Aug 16 1991

ASJC Scopus subject areas

  • Analytical Chemistry
  • Clinical Biochemistry
  • Molecular Medicine

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