Determination of glucocorticoids in UPLC-MS in environmental samples from an occupational setting

Enrico Oddone, Sara Negri, Massimo Bellinzona, Silvia Martino, Marcello Di Tuccio, Elena Grignani, Danilo Cottica, Marcello Imbriani

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Occupational exposures to glucocorticoids are still a neglected issue in some work environments, including pharmaceutical plants. We developed an analytical method to quantify simultaneously 21 glucocorticoids using UPLC coupled with mass spectrometry to provide a basis to carry out environmental monitoring. Samples were taken from air, hand-washing tests, pad-tests and wipe-tests. This paper reports the contents of the analytical methodology, along with the results of this extensive environmental and personal monitoring of glucocorticoids. The method in UPLC-MS turned out to be suitable and effective for the aim of the study. Wipe-test and pad-test desorption was carried out using 50 mL syringes, a simple technique that saves time without adversely affecting analyte recovery. Results showed a widespread environmental pollution due to glucocorticoids. This is of particular concern. Evaluation of the dose absorbed by each worker and identification of a biomarker for occupational exposure will contribute to assessment and prevention of occupational exposure.

Original languageEnglish
Article number678579
JournalInternational Journal of Analytical Chemistry
Publication statusPublished - 2015

ASJC Scopus subject areas

  • Analytical Chemistry

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