Determination of heptylphysostigmine in plasma by high-performance liquid chromatography with electrochemical detection

Luigi Zecca, Davide Radice, Andrea Mosca, Pier Giuseppe Pagella

Research output: Contribution to journalArticle

9 Citations (Scopus)

Abstract

Heptylphysostigmine is a new and very promising cholinergic drug for the treatment of Alzheimer disease. A method has been developed for its determination in plasma with a detection limit of 50 pg/ml. The drug was extracted in n-hexane by a simple one-step procedure, after buffering with sodium bicarbonate. Samples were analysed on a 25 cm × 4.6 mm I.D. silica column (5 μm particle size) using a mixture of acetonitrile, methanol and ammonium nitrate as mobile phase. Since this molecule is quite unstable in plasma, pyridostigmine bromide was added to samples to limit the decomposition. Physostigmine was employed as internal standard. The molecule was electrochemically detected by oxidizing potential (+0.75 V). The method was applied to the analysis of blood samples taken from one healthy volunteer administered this drug. In the same subject the inhibition rate of acetylcholinesterase in plasma and red cells was also measured.

Original languageEnglish
Pages (from-to)169-173
Number of pages5
JournalJournal of Chromatography B: Biomedical Sciences and Applications
Volume615
Issue number1
DOIs
Publication statusPublished - May 19 1993

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High performance liquid chromatography
High Pressure Liquid Chromatography
Plasmas
Pyridostigmine Bromide
Physostigmine
Sodium Bicarbonate
Molecules
Acetylcholinesterase
Plasma Cells
Particle Size
Silicon Dioxide
Pharmaceutical Preparations
Cholinergic Agents
Methanol
Limit of Detection
Healthy Volunteers
Alzheimer Disease
Blood
Particle size
Cells

ASJC Scopus subject areas

  • Chemistry(all)
  • Clinical Biochemistry
  • Molecular Medicine
  • Analytical Chemistry

Cite this

Determination of heptylphysostigmine in plasma by high-performance liquid chromatography with electrochemical detection. / Zecca, Luigi; Radice, Davide; Mosca, Andrea; Pagella, Pier Giuseppe.

In: Journal of Chromatography B: Biomedical Sciences and Applications, Vol. 615, No. 1, 19.05.1993, p. 169-173.

Research output: Contribution to journalArticle

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