Determination of human papillomavirus (HPV) load and type in high-grade cervical lesions surgically resected from HIV-infected women during follow-up of HPV infection

Flavia B. Lillo, Sara Lodini, Davide Ferrari, Carol Stayton, Gianluca Taccagni, Laura Galli, Adriano Lazzarin, Caterina Uberti-Foppa

Research output: Contribution to journalArticle

Abstract

Background. The role of human papillomavirus (HPV) load and the importance of multiple-strain HPV infections as biomarkers for the development of cervical disease were evaluated in human immunodeficiency virus (HIV)-positive women. Methods. A total of 108 samples were analyzed, 64 of which were obtained from 16 HIV-positive women who underwent surgical resection of the cervical cone for treatment of a histologically confirmed high-grade cervical intraepithelial neoplasm (cases) and 44 of which were obtained from 22 HIV-positive women who had high-risk HPV but a negative colposcopy result (controls). Each patient underwent periodic examinations at 6-12-month intervals that included colposcopy, Papanicolaou testing, biopsy (if indicated), and cervical brushing for HPV testing. Viral typing was performed by reverse dot-blot hybridization and quantification of viral load by in-house real-time PCR and commercial assays. Results. Analysis of the cervical-brush samples collected when high-grade squamous intraepithelial lesions were diagnosed revealed that all cases had HPV loads that were significantly higher than those of controls (P = .0004 and P = .0003, by PCR and the Hybrid Capture 2 index [Digene], respectively). Decreasing concentrations of HPV load were observed when comparing samples obtained before and after treatment (P <.0001). The number and type of HPV strains that were detected were not statistically different between cases and controls. Conclusions. The significantly higher HPV load detected in women with high-grade cervical dysplasia, as well as the dramatic decrease in the load after surgical removal of the lesion, suggest that HPV load is a possible prognostic marker of high-grade SIL.

Original languageEnglish
Pages (from-to)451-457
Number of pages7
JournalClinical Infectious Diseases
Volume40
Issue number3
DOIs
Publication statusPublished - Feb 1 2005

Fingerprint

Papillomavirus Infections
HIV
Colposcopy
Uterine Cervical Dysplasia
Cervical Intraepithelial Neoplasia
Viral Load
Real-Time Polymerase Chain Reaction
Biomarkers
Biopsy
Polymerase Chain Reaction

ASJC Scopus subject areas

  • Immunology

Cite this

Determination of human papillomavirus (HPV) load and type in high-grade cervical lesions surgically resected from HIV-infected women during follow-up of HPV infection. / Lillo, Flavia B.; Lodini, Sara; Ferrari, Davide; Stayton, Carol; Taccagni, Gianluca; Galli, Laura; Lazzarin, Adriano; Uberti-Foppa, Caterina.

In: Clinical Infectious Diseases, Vol. 40, No. 3, 01.02.2005, p. 451-457.

Research output: Contribution to journalArticle

@article{97338ec6293942a786695efdb9e1e81f,
title = "Determination of human papillomavirus (HPV) load and type in high-grade cervical lesions surgically resected from HIV-infected women during follow-up of HPV infection",
abstract = "Background. The role of human papillomavirus (HPV) load and the importance of multiple-strain HPV infections as biomarkers for the development of cervical disease were evaluated in human immunodeficiency virus (HIV)-positive women. Methods. A total of 108 samples were analyzed, 64 of which were obtained from 16 HIV-positive women who underwent surgical resection of the cervical cone for treatment of a histologically confirmed high-grade cervical intraepithelial neoplasm (cases) and 44 of which were obtained from 22 HIV-positive women who had high-risk HPV but a negative colposcopy result (controls). Each patient underwent periodic examinations at 6-12-month intervals that included colposcopy, Papanicolaou testing, biopsy (if indicated), and cervical brushing for HPV testing. Viral typing was performed by reverse dot-blot hybridization and quantification of viral load by in-house real-time PCR and commercial assays. Results. Analysis of the cervical-brush samples collected when high-grade squamous intraepithelial lesions were diagnosed revealed that all cases had HPV loads that were significantly higher than those of controls (P = .0004 and P = .0003, by PCR and the Hybrid Capture 2 index [Digene], respectively). Decreasing concentrations of HPV load were observed when comparing samples obtained before and after treatment (P <.0001). The number and type of HPV strains that were detected were not statistically different between cases and controls. Conclusions. The significantly higher HPV load detected in women with high-grade cervical dysplasia, as well as the dramatic decrease in the load after surgical removal of the lesion, suggest that HPV load is a possible prognostic marker of high-grade SIL.",
author = "Lillo, {Flavia B.} and Sara Lodini and Davide Ferrari and Carol Stayton and Gianluca Taccagni and Laura Galli and Adriano Lazzarin and Caterina Uberti-Foppa",
year = "2005",
month = "2",
day = "1",
doi = "10.1086/427032",
language = "English",
volume = "40",
pages = "451--457",
journal = "Clinical Infectious Diseases",
issn = "1058-4838",
publisher = "NLM (Medline)",
number = "3",

}

TY - JOUR

T1 - Determination of human papillomavirus (HPV) load and type in high-grade cervical lesions surgically resected from HIV-infected women during follow-up of HPV infection

AU - Lillo, Flavia B.

AU - Lodini, Sara

AU - Ferrari, Davide

AU - Stayton, Carol

AU - Taccagni, Gianluca

AU - Galli, Laura

AU - Lazzarin, Adriano

AU - Uberti-Foppa, Caterina

PY - 2005/2/1

Y1 - 2005/2/1

N2 - Background. The role of human papillomavirus (HPV) load and the importance of multiple-strain HPV infections as biomarkers for the development of cervical disease were evaluated in human immunodeficiency virus (HIV)-positive women. Methods. A total of 108 samples were analyzed, 64 of which were obtained from 16 HIV-positive women who underwent surgical resection of the cervical cone for treatment of a histologically confirmed high-grade cervical intraepithelial neoplasm (cases) and 44 of which were obtained from 22 HIV-positive women who had high-risk HPV but a negative colposcopy result (controls). Each patient underwent periodic examinations at 6-12-month intervals that included colposcopy, Papanicolaou testing, biopsy (if indicated), and cervical brushing for HPV testing. Viral typing was performed by reverse dot-blot hybridization and quantification of viral load by in-house real-time PCR and commercial assays. Results. Analysis of the cervical-brush samples collected when high-grade squamous intraepithelial lesions were diagnosed revealed that all cases had HPV loads that were significantly higher than those of controls (P = .0004 and P = .0003, by PCR and the Hybrid Capture 2 index [Digene], respectively). Decreasing concentrations of HPV load were observed when comparing samples obtained before and after treatment (P <.0001). The number and type of HPV strains that were detected were not statistically different between cases and controls. Conclusions. The significantly higher HPV load detected in women with high-grade cervical dysplasia, as well as the dramatic decrease in the load after surgical removal of the lesion, suggest that HPV load is a possible prognostic marker of high-grade SIL.

AB - Background. The role of human papillomavirus (HPV) load and the importance of multiple-strain HPV infections as biomarkers for the development of cervical disease were evaluated in human immunodeficiency virus (HIV)-positive women. Methods. A total of 108 samples were analyzed, 64 of which were obtained from 16 HIV-positive women who underwent surgical resection of the cervical cone for treatment of a histologically confirmed high-grade cervical intraepithelial neoplasm (cases) and 44 of which were obtained from 22 HIV-positive women who had high-risk HPV but a negative colposcopy result (controls). Each patient underwent periodic examinations at 6-12-month intervals that included colposcopy, Papanicolaou testing, biopsy (if indicated), and cervical brushing for HPV testing. Viral typing was performed by reverse dot-blot hybridization and quantification of viral load by in-house real-time PCR and commercial assays. Results. Analysis of the cervical-brush samples collected when high-grade squamous intraepithelial lesions were diagnosed revealed that all cases had HPV loads that were significantly higher than those of controls (P = .0004 and P = .0003, by PCR and the Hybrid Capture 2 index [Digene], respectively). Decreasing concentrations of HPV load were observed when comparing samples obtained before and after treatment (P <.0001). The number and type of HPV strains that were detected were not statistically different between cases and controls. Conclusions. The significantly higher HPV load detected in women with high-grade cervical dysplasia, as well as the dramatic decrease in the load after surgical removal of the lesion, suggest that HPV load is a possible prognostic marker of high-grade SIL.

UR - http://www.scopus.com/inward/record.url?scp=13444283305&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=13444283305&partnerID=8YFLogxK

U2 - 10.1086/427032

DO - 10.1086/427032

M3 - Article

C2 - 15668870

AN - SCOPUS:13444283305

VL - 40

SP - 451

EP - 457

JO - Clinical Infectious Diseases

JF - Clinical Infectious Diseases

SN - 1058-4838

IS - 3

ER -