Determination of individual genetic pattern by analysis of hypervariable minisatellites as a new method for establishment of paternity. General considerations and descriptions of a disputed case

C. Romano, M. De Robertis, M. Esposito, G. Ciliberto, A. Pistone, R. Cortese

Research output: Chapter in Book/Report/Conference proceedingChapter

Abstract

In this paper we show the possibility to apply the analysis of the hypervariable minisatellites present in the human genome to the diagnosis of paternity. We take advantage of the recent discovery by Jeffreys and Coll. at the University of Leicester (U.K.) of middle repetitive DNA segments (minisatellites) which show an extreme degree of polymorphism in humans. Making use of particular probes for these regions of the human genome it is possible to establish individual DNA fingerprints of more precise value than usual fingerprints. The polymorphic DNA minisatellites are transmitted stably as mendelian characters through generations and it is possible to demonstrate that the number and length of minisatellites in each individual is inherited in the proportion of 50% from each parent. Here we refer to the use of this new DNA recombinant technology to exclude or to ascertain (prove) with certainty the paternity in a legal contest.

Original languageEnglish
Title of host publicationRivista Italiana di Medicina Legale
Pages234-247
Number of pages14
Volume10
Edition1
Publication statusPublished - 1988

Fingerprint

Paternity
Minisatellite Repeats
Human Genome
Recombinant DNA
DNA Fingerprinting
DNA
Dermatoglyphics
Technology

ASJC Scopus subject areas

  • Medicine(all)

Cite this

Romano, C., De Robertis, M., Esposito, M., Ciliberto, G., Pistone, A., & Cortese, R. (1988). Determination of individual genetic pattern by analysis of hypervariable minisatellites as a new method for establishment of paternity. General considerations and descriptions of a disputed case. In Rivista Italiana di Medicina Legale (1 ed., Vol. 10, pp. 234-247)

Determination of individual genetic pattern by analysis of hypervariable minisatellites as a new method for establishment of paternity. General considerations and descriptions of a disputed case. / Romano, C.; De Robertis, M.; Esposito, M.; Ciliberto, G.; Pistone, A.; Cortese, R.

Rivista Italiana di Medicina Legale. Vol. 10 1. ed. 1988. p. 234-247.

Research output: Chapter in Book/Report/Conference proceedingChapter

Romano, C, De Robertis, M, Esposito, M, Ciliberto, G, Pistone, A & Cortese, R 1988, Determination of individual genetic pattern by analysis of hypervariable minisatellites as a new method for establishment of paternity. General considerations and descriptions of a disputed case. in Rivista Italiana di Medicina Legale. 1 edn, vol. 10, pp. 234-247.
Romano, C. ; De Robertis, M. ; Esposito, M. ; Ciliberto, G. ; Pistone, A. ; Cortese, R. / Determination of individual genetic pattern by analysis of hypervariable minisatellites as a new method for establishment of paternity. General considerations and descriptions of a disputed case. Rivista Italiana di Medicina Legale. Vol. 10 1. ed. 1988. pp. 234-247
@inbook{a40c79e565e24e03a7ec45e2a69e6a25,
title = "Determination of individual genetic pattern by analysis of hypervariable minisatellites as a new method for establishment of paternity. General considerations and descriptions of a disputed case",
abstract = "In this paper we show the possibility to apply the analysis of the hypervariable minisatellites present in the human genome to the diagnosis of paternity. We take advantage of the recent discovery by Jeffreys and Coll. at the University of Leicester (U.K.) of middle repetitive DNA segments (minisatellites) which show an extreme degree of polymorphism in humans. Making use of particular probes for these regions of the human genome it is possible to establish individual DNA fingerprints of more precise value than usual fingerprints. The polymorphic DNA minisatellites are transmitted stably as mendelian characters through generations and it is possible to demonstrate that the number and length of minisatellites in each individual is inherited in the proportion of 50{\%} from each parent. Here we refer to the use of this new DNA recombinant technology to exclude or to ascertain (prove) with certainty the paternity in a legal contest.",
author = "C. Romano and {De Robertis}, M. and M. Esposito and G. Ciliberto and A. Pistone and R. Cortese",
year = "1988",
language = "English",
volume = "10",
pages = "234--247",
booktitle = "Rivista Italiana di Medicina Legale",
edition = "1",

}

TY - CHAP

T1 - Determination of individual genetic pattern by analysis of hypervariable minisatellites as a new method for establishment of paternity. General considerations and descriptions of a disputed case

AU - Romano, C.

AU - De Robertis, M.

AU - Esposito, M.

AU - Ciliberto, G.

AU - Pistone, A.

AU - Cortese, R.

PY - 1988

Y1 - 1988

N2 - In this paper we show the possibility to apply the analysis of the hypervariable minisatellites present in the human genome to the diagnosis of paternity. We take advantage of the recent discovery by Jeffreys and Coll. at the University of Leicester (U.K.) of middle repetitive DNA segments (minisatellites) which show an extreme degree of polymorphism in humans. Making use of particular probes for these regions of the human genome it is possible to establish individual DNA fingerprints of more precise value than usual fingerprints. The polymorphic DNA minisatellites are transmitted stably as mendelian characters through generations and it is possible to demonstrate that the number and length of minisatellites in each individual is inherited in the proportion of 50% from each parent. Here we refer to the use of this new DNA recombinant technology to exclude or to ascertain (prove) with certainty the paternity in a legal contest.

AB - In this paper we show the possibility to apply the analysis of the hypervariable minisatellites present in the human genome to the diagnosis of paternity. We take advantage of the recent discovery by Jeffreys and Coll. at the University of Leicester (U.K.) of middle repetitive DNA segments (minisatellites) which show an extreme degree of polymorphism in humans. Making use of particular probes for these regions of the human genome it is possible to establish individual DNA fingerprints of more precise value than usual fingerprints. The polymorphic DNA minisatellites are transmitted stably as mendelian characters through generations and it is possible to demonstrate that the number and length of minisatellites in each individual is inherited in the proportion of 50% from each parent. Here we refer to the use of this new DNA recombinant technology to exclude or to ascertain (prove) with certainty the paternity in a legal contest.

UR - http://www.scopus.com/inward/record.url?scp=0023768422&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=0023768422&partnerID=8YFLogxK

M3 - Chapter

AN - SCOPUS:0023768422

VL - 10

SP - 234

EP - 247

BT - Rivista Italiana di Medicina Legale

ER -