Determination of linezolid in human plasma by high-performance liquid chromatography with ultraviolet detection

Dario Cattaneo, Sara Baldelli, Francesca Conti, Valeria Cozzi, Emilio Clementi

Research output: Contribution to journalArticlepeer-review

Abstract

A high-performance liquid chromatographic method for the determination of linezolid in human plasma was developed and validated. After precipitation of plasma proteins with perchloric acid, the protein-free supernatant was separated by isocratic reverse-phase chromatography on a X Bridge C18 column. The mobile phase consisted of a mixture of phosphoric acid 0.05%: acetonitrile (75:25, v/v) with a flow rate of 1 mL/min. The column elute was monitored at 254 nm. The method was linear from 0.2 to 48 mg/L (mean r = 0.9996, n = 10). The observed intra-and inter-day assay imprecision ranged from 2.83% to 8.16% (18.80% at the lower limit of quantification); inaccuracy varied between-0.33% and 8.18%. Mean drug recovery was 99.8% for linezolid and 90.0% for the internal standard (para-toluic acid). The method was found to be precise and accurate and suitable for therapeutic drug monitoring of linezolid in routine clinical practice.

Original languageEnglish
Pages (from-to)520-524
Number of pages5
JournalTherapeutic Drug Monitoring
Volume32
Issue number4
DOIs
Publication statusPublished - Aug 2010

Keywords

  • HPLC
  • human plasma
  • linezolid
  • pharmacokinetics
  • therapeutic drug monitoring

ASJC Scopus subject areas

  • Pharmacology (medical)
  • Pharmacology
  • Medicine(all)

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