Determination of methotrexate in human urine at trace levels by solid phase extraction and high-performance liquid chromatography/tandem mass spectrometry

Roberta Turci, Maria Lorena Fiorentino, Cristina Sottani, Claudio Minoia

Research output: Contribution to journalArticlepeer-review

Abstract

For biological monitoring of hospital personnel occupationally exposed to antineoplastic agents, highly sensitive and specific methods are required. In order to detect trace MTX urinary concentrations, a precise and accurate high-performance liquid chromatography/tandem mass spectrometry (HPLC-MS/MS) procedure, incorporating solid phase extraction, has been developed. Urine samples were purified by solid phase extraction (SPE) on octadecyl bonded, endcapped silica SPE columns. After eluting with methanol, the solvent was evaporated obtaining a 25-fold concentration of the analyte. This procedure was validated by using 7-OHMTX as internal standard. Calibration curves had correlation coefficients always higher than 0.999, and the limit of detection was assessed at 0.2 μg L-1. High specificity of the HPLC-MS/MS technique assures that no interfering substances are detected rather than the analyte of interest. (C) 2000 John Wiley and Sons, Ltd.

Original languageEnglish
Pages (from-to)173-179
Number of pages7
JournalRapid Communications in Mass Spectrometry
Volume14
Issue number3
Publication statusPublished - 2000

ASJC Scopus subject areas

  • Analytical Chemistry
  • Spectroscopy

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