Determination of monobromobimane derivatives of phenylmercapturic and benzylmercapturic acids in urine by high-performance liquid chromatography and fluorimetry

M. Buratti, G. Brambilla, S. Fustinoni, O. Pellegrino, S. Pulviremti, A. Colombi

Research output: Contribution to journalArticlepeer-review

Abstract

A method was developed for the determination in human urine of S-phenylmercapturic (PMA) and S-benzylmercapturic (BMA) acids, metabolites respectively of benzene and toluene. PMA and BMA were determined, after alkaline hydrolysis, to give respectively thiophenol and benzylmercaptan, and coupling of the thiol-containing compounds with monobromobimane (MB), by reversed-phase HPLC on a diphenyl-silica bonded cartridge (100×4.6 mm I.D., 5 μm particle size) with fluorimetric detection. Wavelengths for excitation and emission were 375 and 480 nm, respectively. The recovery of PMA and BMA from spiked urines was >90% in the 10-500 μg/l range; the quantification limits were respectively 1 and 0.5 μg/l; day-to-day precision at 42 μg/l was C.V. 3) according to the equation y=6.5+0.65x (r=0.69, P

Original languageEnglish
Pages (from-to)305-313
Number of pages9
JournalJournal of Chromatography B: Biomedical Sciences and Applications
Volume751
Issue number2
DOIs
Publication statusPublished - Feb 25 2001

Keywords

  • Benzene
  • Benzylmercapturic acid
  • Exposure biomarkers
  • Phenylmercapturic acid
  • Tobacco smoking
  • Toluene

ASJC Scopus subject areas

  • Chemistry(all)

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