Abstract
Perampanel (PMP) (2- [2-oxo-1-phenyl-5-pyridin-2-y1-1,2 dihydropyridin-3-y1] benzonitrile hydrate) is a novel non-competitive selective antagonist of the postsynaptic ionotropic alpha-amino-3-hydroxy-5-methyl-4-isoxazoleproprionic acid (AMPA) glutamate receptor. In this study, we compared two different methods for the quantification of perampanel in human plasma: a commercially available HPLC kit with fluorescent detector and a kit for determination of 18 different antiepileptic drugs (perampanel excluded) based on liquid chromatography-tandem mass spectrometry (LC-MS/MS) used to implement the perampanel determination. LC-MS/MS method was fully validated according to available guidelines: calibration curves were linear across perampanel concentration range of 2,5-2,800 ng/mL with correlation coefficient >0,99. Accuracy and precision results were in accordance with acceptance criteria (15%). Data collected from 95 patients, treated with perampanel as maintenance antiepileptic therapy, show a very strong correlation between the two methods. Perampanel plasma concentrations in patient samples at steady state, determined with the LC-MS/MS method, ranged from 35 to 985 ng/mL and are in line with previously reported data.
| Original language | Italian |
|---|---|
| Pages (from-to) | 217-224 |
| Number of pages | 8 |
| Journal | Biochimica Clinica |
| Volume | 42 |
| Issue number | 3 |
| DOIs | |
| Publication status | Published - Jan 1 2018 |
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ASJC Scopus subject areas
- Clinical Biochemistry
- Medical Laboratory Technology
- Biochemistry, medical
Cite this
Determinazione del perampanel plasmatico : confronto e cross-validazione tra due metodi in cromatografia liquida ad elevate prestazioni con rivelatore spettrofluorimetrico e con rivelatore a spettrometria di massa. / D'Urso, Annachiara; Fontana, Federica; Gabrieletto, Ivana; Gilardoni, Francesca; De Riva, Valentina; Contarato, Giorgia; Ranzato, Federica; Galloni, Elisabetta; De Grazia, Ugo.
In: Biochimica Clinica, Vol. 42, No. 3, 01.01.2018, p. 217-224.Research output: Contribution to journal › Article
}
TY - JOUR
T1 - Determinazione del perampanel plasmatico
T2 - confronto e cross-validazione tra due metodi in cromatografia liquida ad elevate prestazioni con rivelatore spettrofluorimetrico e con rivelatore a spettrometria di massa
AU - D'Urso, Annachiara
AU - Fontana, Federica
AU - Gabrieletto, Ivana
AU - Gilardoni, Francesca
AU - De Riva, Valentina
AU - Contarato, Giorgia
AU - Ranzato, Federica
AU - Galloni, Elisabetta
AU - De Grazia, Ugo
PY - 2018/1/1
Y1 - 2018/1/1
N2 - Perampanel (PMP) (2- [2-oxo-1-phenyl-5-pyridin-2-y1-1,2 dihydropyridin-3-y1] benzonitrile hydrate) is a novel non-competitive selective antagonist of the postsynaptic ionotropic alpha-amino-3-hydroxy-5-methyl-4-isoxazoleproprionic acid (AMPA) glutamate receptor. In this study, we compared two different methods for the quantification of perampanel in human plasma: a commercially available HPLC kit with fluorescent detector and a kit for determination of 18 different antiepileptic drugs (perampanel excluded) based on liquid chromatography-tandem mass spectrometry (LC-MS/MS) used to implement the perampanel determination. LC-MS/MS method was fully validated according to available guidelines: calibration curves were linear across perampanel concentration range of 2,5-2,800 ng/mL with correlation coefficient >0,99. Accuracy and precision results were in accordance with acceptance criteria (15%). Data collected from 95 patients, treated with perampanel as maintenance antiepileptic therapy, show a very strong correlation between the two methods. Perampanel plasma concentrations in patient samples at steady state, determined with the LC-MS/MS method, ranged from 35 to 985 ng/mL and are in line with previously reported data.
AB - Perampanel (PMP) (2- [2-oxo-1-phenyl-5-pyridin-2-y1-1,2 dihydropyridin-3-y1] benzonitrile hydrate) is a novel non-competitive selective antagonist of the postsynaptic ionotropic alpha-amino-3-hydroxy-5-methyl-4-isoxazoleproprionic acid (AMPA) glutamate receptor. In this study, we compared two different methods for the quantification of perampanel in human plasma: a commercially available HPLC kit with fluorescent detector and a kit for determination of 18 different antiepileptic drugs (perampanel excluded) based on liquid chromatography-tandem mass spectrometry (LC-MS/MS) used to implement the perampanel determination. LC-MS/MS method was fully validated according to available guidelines: calibration curves were linear across perampanel concentration range of 2,5-2,800 ng/mL with correlation coefficient >0,99. Accuracy and precision results were in accordance with acceptance criteria (15%). Data collected from 95 patients, treated with perampanel as maintenance antiepileptic therapy, show a very strong correlation between the two methods. Perampanel plasma concentrations in patient samples at steady state, determined with the LC-MS/MS method, ranged from 35 to 985 ng/mL and are in line with previously reported data.
UR - http://www.scopus.com/inward/record.url?scp=85054870130&partnerID=8YFLogxK
UR - http://www.scopus.com/inward/citedby.url?scp=85054870130&partnerID=8YFLogxK
U2 - 10.19186/BC_2018.042
DO - 10.19186/BC_2018.042
M3 - Articolo
AN - SCOPUS:85054870130
VL - 42
SP - 217
EP - 224
JO - Biochimica Clinica
JF - Biochimica Clinica
SN - 0393-0564
IS - 3
ER -