Determinazione del perampanel plasmatico: confronto e cross-validazione tra due metodi in cromatografia liquida ad elevate prestazioni con rivelatore spettrofluorimetrico e con rivelatore a spettrometria di massa

Translated title of the contribution: Determination of perampanel in plasma samples: Comparison and cross-validation between two HPLC methods utilizing two different detectors: Spectrofluorimetric and mass spectrometry

Annachiara D'Urso, Federica Fontana, Ivana Gabrieletto, Francesca Gilardoni, Valentina De Riva, Giorgia Contarato, Federica Ranzato, Elisabetta Galloni, Ugo De Grazia

Research output: Contribution to journalArticle

Abstract

Perampanel (PMP) (2- [2-oxo-1-phenyl-5-pyridin-2-y1-1,2 dihydropyridin-3-y1] benzonitrile hydrate) is a novel non-competitive selective antagonist of the postsynaptic ionotropic alpha-amino-3-hydroxy-5-methyl-4-isoxazoleproprionic acid (AMPA) glutamate receptor. In this study, we compared two different methods for the quantification of perampanel in human plasma: a commercially available HPLC kit with fluorescent detector and a kit for determination of 18 different antiepileptic drugs (perampanel excluded) based on liquid chromatography-tandem mass spectrometry (LC-MS/MS) used to implement the perampanel determination. LC-MS/MS method was fully validated according to available guidelines: calibration curves were linear across perampanel concentration range of 2,5-2,800 ng/mL with correlation coefficient >0,99. Accuracy and precision results were in accordance with acceptance criteria (15%). Data collected from 95 patients, treated with perampanel as maintenance antiepileptic therapy, show a very strong correlation between the two methods. Perampanel plasma concentrations in patient samples at steady state, determined with the LC-MS/MS method, ranged from 35 to 985 ng/mL and are in line with previously reported data.

Original languageItalian
Pages (from-to)217-224
Number of pages8
JournalBiochimica Clinica
Volume42
Issue number3
DOIs
Publication statusPublished - Jan 1 2018

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Mass spectrometry
Mass Spectrometry
High Pressure Liquid Chromatography
Detectors
Plasmas
Anticonvulsants
Plasma (human)
alpha-Amino-3-hydroxy-5-methyl-4-isoxazolepropionic Acid
AMPA Receptors
Liquid chromatography
Glutamate Receptors
Tandem Mass Spectrometry
perampanel
Hydrates
Liquid Chromatography
Calibration
Maintenance
Guidelines
Acids

ASJC Scopus subject areas

  • Clinical Biochemistry
  • Medical Laboratory Technology
  • Biochemistry, medical

Cite this

Determinazione del perampanel plasmatico : confronto e cross-validazione tra due metodi in cromatografia liquida ad elevate prestazioni con rivelatore spettrofluorimetrico e con rivelatore a spettrometria di massa. / D'Urso, Annachiara; Fontana, Federica; Gabrieletto, Ivana; Gilardoni, Francesca; De Riva, Valentina; Contarato, Giorgia; Ranzato, Federica; Galloni, Elisabetta; De Grazia, Ugo.

In: Biochimica Clinica, Vol. 42, No. 3, 01.01.2018, p. 217-224.

Research output: Contribution to journalArticle

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