Determination of tebuconazole and penconazole fungicides in rat and human hair by liquid chromatography/tandem mass spectrometry

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6 Citations (Scopus)

Abstract

Rationale: Tebuconazole (TEB) and penconazole (PEN) are widely applied fungicides and environmental contaminants; their toxicological properties include possible effects to the unborn child, therefore, the evaluation of human exposure is relevant to risk assessment. Hair is a non-invasive specimen that incorporates pollutants allowing an extended exposure window to be surveyed. The aim of this work was to develop and validate an assay for the determination of TEB and PEN in human hair. Methods: Under optimised conditions, analytes were extracted by soaking hair in acetonitrile, in the presence of deuterated analogues, under heating and agitation. Chemical separation was achieved using a C18 reversed-phase chromatographic column and detection and quantification were performed, after positive electrospray ionization, by triple quadrupole mass spectrometry operating in selected reaction monitoring mode. Results: The assay validation showed a linear dynamic range up to 5 μg/L or 200 pg/mg hair, inter- and intra-run precisions <6%, and accuracies within 5% of spiked concentrations. Limits of quantification were 0.001 μg/L or 1 pg/mg hair for both TEB and PEN. Matrix effect experiments showed that the isotope dilution approach allowed for the control of bias sources. TEB and PEN were determined in hair of rats exposed to a low dose of TEB and in hair of agricultural workers exposed to TEB and/or PEN during the application season, indicating that both chemicals are incorporated into the hair upon exposure. Conclusions: The results of this study indicate that the developed assay is useful to evaluate the exposure to TEB and PEN in humans.

Original languageEnglish
Pages (from-to)1243-1249
Number of pages7
JournalRapid Communications in Mass Spectrometry
Volume32
Issue number15
DOIs
Publication statusPublished - Aug 15 2018

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Fungicides
Liquid chromatography
Mass spectrometry
Rats
Assays
Electrospray ionization
penconazole
tebuconazole
Isotopes
Risk assessment
Dilution
Impurities
Heating
Monitoring

ASJC Scopus subject areas

  • Analytical Chemistry
  • Spectroscopy
  • Organic Chemistry

Cite this

@article{8bc4f01aa67442baa7f3a5ad79418d77,
title = "Determination of tebuconazole and penconazole fungicides in rat and human hair by liquid chromatography/tandem mass spectrometry",
abstract = "Rationale: Tebuconazole (TEB) and penconazole (PEN) are widely applied fungicides and environmental contaminants; their toxicological properties include possible effects to the unborn child, therefore, the evaluation of human exposure is relevant to risk assessment. Hair is a non-invasive specimen that incorporates pollutants allowing an extended exposure window to be surveyed. The aim of this work was to develop and validate an assay for the determination of TEB and PEN in human hair. Methods: Under optimised conditions, analytes were extracted by soaking hair in acetonitrile, in the presence of deuterated analogues, under heating and agitation. Chemical separation was achieved using a C18 reversed-phase chromatographic column and detection and quantification were performed, after positive electrospray ionization, by triple quadrupole mass spectrometry operating in selected reaction monitoring mode. Results: The assay validation showed a linear dynamic range up to 5 μg/L or 200 pg/mg hair, inter- and intra-run precisions <6{\%}, and accuracies within 5{\%} of spiked concentrations. Limits of quantification were 0.001 μg/L or 1 pg/mg hair for both TEB and PEN. Matrix effect experiments showed that the isotope dilution approach allowed for the control of bias sources. TEB and PEN were determined in hair of rats exposed to a low dose of TEB and in hair of agricultural workers exposed to TEB and/or PEN during the application season, indicating that both chemicals are incorporated into the hair upon exposure. Conclusions: The results of this study indicate that the developed assay is useful to evaluate the exposure to TEB and PEN in humans.",
author = "Elisa Polledri and Rosa Mercadante and Silvia Fustinoni",
year = "2018",
month = "8",
day = "15",
doi = "10.1002/rcm.8153",
language = "English",
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pages = "1243--1249",
journal = "Rapid Communications in Mass Spectrometry",
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T1 - Determination of tebuconazole and penconazole fungicides in rat and human hair by liquid chromatography/tandem mass spectrometry

AU - Polledri, Elisa

AU - Mercadante, Rosa

AU - Fustinoni, Silvia

PY - 2018/8/15

Y1 - 2018/8/15

N2 - Rationale: Tebuconazole (TEB) and penconazole (PEN) are widely applied fungicides and environmental contaminants; their toxicological properties include possible effects to the unborn child, therefore, the evaluation of human exposure is relevant to risk assessment. Hair is a non-invasive specimen that incorporates pollutants allowing an extended exposure window to be surveyed. The aim of this work was to develop and validate an assay for the determination of TEB and PEN in human hair. Methods: Under optimised conditions, analytes were extracted by soaking hair in acetonitrile, in the presence of deuterated analogues, under heating and agitation. Chemical separation was achieved using a C18 reversed-phase chromatographic column and detection and quantification were performed, after positive electrospray ionization, by triple quadrupole mass spectrometry operating in selected reaction monitoring mode. Results: The assay validation showed a linear dynamic range up to 5 μg/L or 200 pg/mg hair, inter- and intra-run precisions <6%, and accuracies within 5% of spiked concentrations. Limits of quantification were 0.001 μg/L or 1 pg/mg hair for both TEB and PEN. Matrix effect experiments showed that the isotope dilution approach allowed for the control of bias sources. TEB and PEN were determined in hair of rats exposed to a low dose of TEB and in hair of agricultural workers exposed to TEB and/or PEN during the application season, indicating that both chemicals are incorporated into the hair upon exposure. Conclusions: The results of this study indicate that the developed assay is useful to evaluate the exposure to TEB and PEN in humans.

AB - Rationale: Tebuconazole (TEB) and penconazole (PEN) are widely applied fungicides and environmental contaminants; their toxicological properties include possible effects to the unborn child, therefore, the evaluation of human exposure is relevant to risk assessment. Hair is a non-invasive specimen that incorporates pollutants allowing an extended exposure window to be surveyed. The aim of this work was to develop and validate an assay for the determination of TEB and PEN in human hair. Methods: Under optimised conditions, analytes were extracted by soaking hair in acetonitrile, in the presence of deuterated analogues, under heating and agitation. Chemical separation was achieved using a C18 reversed-phase chromatographic column and detection and quantification were performed, after positive electrospray ionization, by triple quadrupole mass spectrometry operating in selected reaction monitoring mode. Results: The assay validation showed a linear dynamic range up to 5 μg/L or 200 pg/mg hair, inter- and intra-run precisions <6%, and accuracies within 5% of spiked concentrations. Limits of quantification were 0.001 μg/L or 1 pg/mg hair for both TEB and PEN. Matrix effect experiments showed that the isotope dilution approach allowed for the control of bias sources. TEB and PEN were determined in hair of rats exposed to a low dose of TEB and in hair of agricultural workers exposed to TEB and/or PEN during the application season, indicating that both chemicals are incorporated into the hair upon exposure. Conclusions: The results of this study indicate that the developed assay is useful to evaluate the exposure to TEB and PEN in humans.

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