TY - JOUR
T1 - Determination of terbuthylazine and desethylterbuthylazine in human urine and hair samples by eletrospray ionization-liquid chromatography/triple quadrupole mass spectrometry
AU - Mercadante, Rosa
AU - Polledri, Elisa
AU - Fustinoni, Silvia
PY - 2012/8
Y1 - 2012/8
N2 - Terbuthylazine (TBA) is a widely applied herbicide and an environmental contaminant. Following its use, humans, such as agricultural workers and rural residents, may be exposed. An isotope-dilution liquid chromatography coupled to electrospray-tandem mass spectrometry method for the determination of TBA, and its metabolite desethylterbuthylazine (DET) in human urine and hair was developed and validated. Under the optimised conditions, analytes were extracted from urine using a solid phase cartridge or from hair by sonication in methanol. Analytes were separated using a C18 reversed-phase chromatographic column and quantified, after positive ionization using a heated electrospray source, by a triple quadrupole mass detector in the selected reaction monitoring mode. Validation showed linear dynamic ranges up to 100 μg/L or 5.00 ng/mg hair, inter-and intra-run precisions
AB - Terbuthylazine (TBA) is a widely applied herbicide and an environmental contaminant. Following its use, humans, such as agricultural workers and rural residents, may be exposed. An isotope-dilution liquid chromatography coupled to electrospray-tandem mass spectrometry method for the determination of TBA, and its metabolite desethylterbuthylazine (DET) in human urine and hair was developed and validated. Under the optimised conditions, analytes were extracted from urine using a solid phase cartridge or from hair by sonication in methanol. Analytes were separated using a C18 reversed-phase chromatographic column and quantified, after positive ionization using a heated electrospray source, by a triple quadrupole mass detector in the selected reaction monitoring mode. Validation showed linear dynamic ranges up to 100 μg/L or 5.00 ng/mg hair, inter-and intra-run precisions
KW - Biological monitoring
KW - Desethylterbuthylazine
KW - Hair
KW - Terbuthylazine
KW - Urine
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U2 - 10.1007/s00216-012-6184-3
DO - 10.1007/s00216-012-6184-3
M3 - Article
C2 - 22752446
AN - SCOPUS:84865459139
VL - 404
SP - 875
EP - 886
JO - Fresenius Journal of Analytical Chemistry
JF - Fresenius Journal of Analytical Chemistry
SN - 0016-1152
IS - 3
ER -