Determination of terbuthylazine and desethylterbuthylazine in human urine and hair samples by eletrospray ionization-liquid chromatography/triple quadrupole mass spectrometry

Rosa Mercadante, Elisa Polledri, Silvia Fustinoni

Research output: Contribution to journalArticle

Abstract

Terbuthylazine (TBA) is a widely applied herbicide and an environmental contaminant. Following its use, humans, such as agricultural workers and rural residents, may be exposed. An isotope-dilution liquid chromatography coupled to electrospray-tandem mass spectrometry method for the determination of TBA, and its metabolite desethylterbuthylazine (DET) in human urine and hair was developed and validated. Under the optimised conditions, analytes were extracted from urine using a solid phase cartridge or from hair by sonication in methanol. Analytes were separated using a C18 reversed-phase chromatographic column and quantified, after positive ionization using a heated electrospray source, by a triple quadrupole mass detector in the selected reaction monitoring mode. Validation showed linear dynamic ranges up to 100 μg/L or 5.00 ng/mg hair, inter-and intra-run precisions

Original languageEnglish
Pages (from-to)875-886
Number of pages12
JournalAnalytical and Bioanalytical Chemistry
Volume404
Issue number3
DOIs
Publication statusPublished - Aug 2012

Keywords

  • Biological monitoring
  • Desethylterbuthylazine
  • Hair
  • Terbuthylazine
  • Urine

ASJC Scopus subject areas

  • Analytical Chemistry
  • Biochemistry

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