Determination of total and hexavalent chromium in bile after intravenous administration of potassium dichromate in rats

Alessandro Cavalleri, Claudio Minoia, Plinio Richelmi, Carla Baldi, Guiseppina Micoli

Research output: Contribution to journalArticle

Abstract

Total and hexavalent chromium were measured in bile samples obtained from cannulated bile ducts of male rats iv administered with potassium dichromate at various doses corresponding to 0.1, 0.5, and 1 mg of chromium. The evaluation of the hexavalent form was performed by separation with a liquid anion exchanger and electrothermal atomization-atomic absorption spectrophotometric determination. Within 2 hr 1.35-2.23% of the chromium injected was recovered in bile as total chromium, the hexavalent form accounting for less than 1% of the total chromium collected, which seems almost entirely excreted as trivalent chromium. Since Cr(VI) administered iv was quickly reduced to Cr(III) in blood, the possibility exists for chromium in trivalent form to penetrate into the liver cells and to be excreted in the bile, possibly by binding to a carrier such as the low-molecular-weight substance described by Yamamoto et al. (A. Yamamoto, O. Wada, and T. Ono, Toxicol. Appl. Pharmacol., 59, 515, 1981).

Original languageEnglish
Pages (from-to)490-496
Number of pages7
JournalEnvironmental Research
Volume37
Issue number2
DOIs
Publication statusPublished - 1985

ASJC Scopus subject areas

  • Environmental Science(all)

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