Determination of urinary ortho- and meta-cresol in humans by headspace SPME gas chromatography/mass spectrometry

Silvia Fustinoni, Rosa Mercadante, Laura Campo, Licia Scibetta, Carla Valla, Vito Foà

Research output: Contribution to journalArticle

Abstract

ortho-Cresol (o-C) and meta-cresol (m-C) are minor urinary metabolites of toluene, a widely used chemical with neurotoxicological properties. A new assay for their determination in human urine is here proposed. Urinary cresol sulphates and glucuronates are submitted to acid hydrolysis, urine is neutralized, added with o-cresols-d8, and analytes are sampled in the headspace of urine by SPME using a polydimethylsiloxane fiber. Analysis is performed by GC/MS using, for separation, either a SupelcoWax10 (for o-C) or a chiral CP Cresol (for o-C and m-C) column. The method is very specific, with a range of linearity 0-5.0 mg/l, within- and between-run precision, as coefficient of variation,

Original languageEnglish
Pages (from-to)309-317
Number of pages9
JournalJournal of Chromatography B: Analytical Technologies in the Biomedical and Life Sciences
Volume817
Issue number2
DOIs
Publication statusPublished - Mar 25 2005

Keywords

  • Biological monitoring
  • Occupational exposure to toluene
  • Urinary cresols

ASJC Scopus subject areas

  • Biochemistry

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