Detetermination of reference values for a colorimetric fructosamine assay

J. Henny, F. Schiele, J. D. Kruse-Jarres, C. Kaiser, P. Bonini, F. Ceriotti, H. Schlebusch, M. Sorger, L. Garcia-Beltran, J. Jarausch, G. Klein, B. W. Vogt, B. Zawta, I. Domke

Research output: Contribution to journalArticle

Abstract

The study was aimed at establishing reference values for an improved fructosamine assay based on a new standardization protocol. Fructosamine values were determined in sera of 1047 non-diabetic healthy adults of both sexes, 20 to 60 years old in five laboratories on different clinical chemistry analyzers as well as in 603 children (aged 4 to 19 years) in one laboratory and in 52 newborns and 252 children (aged 1 month to 18 years) in another laboratory. Fructosamine concentrations were also measured in the sera of 340 pregnant women and in 179 diabetic patients with unselected insulin-dependent and non-insulin-dependent diabetes mellitus. The reference range in adults is 203 to 285 μmol/l (2.5th to 97.5th percentile). The influence of sex on fructosamine values is negligible. Newborns have lower fructosamine values (median 199 μmol/l). Fructosamine concentrations increase slightly from childhood to adolescence. This increase is abolished if the fructosamine value is related to total protein. With progressing pregnancy the serum fructosamine concentration decreases. Relation to total protein leads to fructosamine values which are independent of the gestational age. Fructosamine is increased in diabetics (median 354 μmol/l) compared to values in the reference group (median 243 μmol/l). The simple, quick and fully automated fructosamine method is very suitable for integration into the routine management of diabetic patients.

Original languageEnglish
Pages (from-to)153-160
Number of pages8
JournalKlinisches Labor
Volume38
Issue number4
Publication statusPublished - 1992

Keywords

  • children and pregnant women
  • fructosamine
  • glycated serum proteins
  • reference values for adults

ASJC Scopus subject areas

  • Biochemistry, Genetics and Molecular Biology(all)

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    Henny, J., Schiele, F., Kruse-Jarres, J. D., Kaiser, C., Bonini, P., Ceriotti, F., Schlebusch, H., Sorger, M., Garcia-Beltran, L., Jarausch, J., Klein, G., Vogt, B. W., Zawta, B., & Domke, I. (1992). Detetermination of reference values for a colorimetric fructosamine assay. Klinisches Labor, 38(4), 153-160.