Development and evaluation of a decellularized membrane from human dermis

Elena Bondioli, Milena Fini, Francesca Veronesi, Gianluca Giavaresi, Matilde Tschon, Giovanna Cenacchi, Serenella Cerasoli, Roberto Giardino, Davide Melandri

Research output: Contribution to journalArticlepeer-review


Interest is increasing in biological scaffolds for tissue regeneration, such as extracellular matrix (ECM) membranes, developed through soft tissue decellularization. The present study describes the development of a chemicophysical decellularization method applied to allogenic human-derived dermis (HDM). To evaluate the absence of viable cells and the maintenance of ECM structure, biological, histological and ultrastructural assessments were performed on the HDM membrane. Residual DNA content and glycosaminoglycan (GAG) and collagen contents were quantified. Growth factor (GF) release was directly measured on HDM extracts and indirectly measured by assessing cell proliferation after administering extract to cultures. Tensile tests were performed to measure the effect of the decellularization technique on the mechanical properties of tissue. Histocompatibility was investigated after subcutaneous implantation in rats. Residual DNA, GAG and collagen content measurements, vitality index, histology and electron microscopy showed the efficiency of the decellularization process and preservation of ECM matrix and bioactivity. In HDM extracts, among the tested GFs, transforming growth factor-β1 showed the highest concentration. HDM extracts significantly increased the proliferation rate of L929 fibroblasts in comparison with controls (p

Original languageEnglish
Pages (from-to)325-336
Number of pages12
JournalJournal of Tissue Engineering and Regenerative Medicine
Issue number4
Publication statusPublished - 2014


  • Bioactivity
  • Collagenous membrane
  • Decellularization
  • Extracellular matrix
  • Human dermis
  • Scaffold

ASJC Scopus subject areas

  • Biomedical Engineering
  • Medicine (miscellaneous)
  • Biomaterials
  • Medicine(all)


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