Development and mechanical characterization of a collagen/hydroxyapatite bilayered scaffold for ostechondral defect replacement

Francesca Gervaso, Francesca Scalera, Sanosh Kunjalukkal Padmanabhan, Antonio Licciulli, Daniela Deponti, Alessia Di Giancamillo, Cinzia Domeneghini, Giuseppe M. Peretti, Alessandro Sannino

Research output: Chapter in Book/Report/Conference proceedingConference contribution

Abstract

In this work a novel three-dimensional ostechondral substitute is proposed that is made of an inorganic/organic hybrid material, namely collagen/hydroxyapatite. The two components of the substitute have been characterized separately. The inorganic part, a hydroxyapatite scaffold, was fabricated by a polymer sponge templating method using a reactive sub-micron powder synthesized in our laboratory by hydroxide precipitation sol-gel route. The organic part, a collagen scaffold, was fabricated by a freeze-dying technique varying design parameters. Both the parts were analysed by scanning electron microscopy and their mechanical properties assessed by compression tests. The hydroxyapatite scaffold showed a high and highly interconnected porosity and a mechanical strength equal to 0.55 MPa, higher than those reported in literature. The collagen scaffolds were seeded by chondrocytes, processed for histology analysis and tested in compression. The biological tests proved the ability of the scaffolds to be positively populated by chondrocytes and the mechanical analysis showed that the mechanical strength of the scaffolds significantly increased after 3 weeks of culture.

Original languageEnglish
Title of host publicationKey Engineering Materials
Pages890-895
Number of pages6
Volume493-494
DOIs
Publication statusPublished - 2012
Event23rd Symposium and Annual Meeting of International Society for Ceramics in Medicine, ISCM 2011 - Istanbul, Turkey
Duration: Nov 6 2011Nov 9 2011

Publication series

NameKey Engineering Materials
Volume493-494
ISSN (Print)10139826

Other

Other23rd Symposium and Annual Meeting of International Society for Ceramics in Medicine, ISCM 2011
CountryTurkey
CityIstanbul
Period11/6/1111/9/11

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Keywords

  • Biological test
  • Bone
  • Cartilage
  • Compression test
  • Tissue engineering

ASJC Scopus subject areas

  • Materials Science(all)
  • Mechanics of Materials
  • Mechanical Engineering

Cite this

Gervaso, F., Scalera, F., Padmanabhan, S. K., Licciulli, A., Deponti, D., Giancamillo, A. D., Domeneghini, C., Peretti, G. M., & Sannino, A. (2012). Development and mechanical characterization of a collagen/hydroxyapatite bilayered scaffold for ostechondral defect replacement. In Key Engineering Materials (Vol. 493-494, pp. 890-895). (Key Engineering Materials; Vol. 493-494). https://doi.org/10.4028/www.scientific.net/KEM.493-494.890