Abstract
This paper analyzes the effects of exogenously supplied GM1 on the development, i.e., specific neurotransmitter uptake capability and survival, of the dopaminergic neurons present in fetal mouse-dissociated mesencephalic cells. Exogenous GM1, but not asialo-GM1, sialic acid, or the oligosaccharide chain of GM1, sialic acid, or the oligosaccharide chain of GM1, enhances in a time- and concentration-dependent manner the specific 3H-dopamine uptake (increase of the apparent V(max) and decrease of the apparent K(m) value) and the long-term survival of the dopaminergic neurons. The GM1 effects on the behavior of the dopaminergic neurons require the presence of cell-derived neuronotrophic influences present within the culture system and are associated with an increase in the response of the cells to the trophic influences. GM1 effects are not limited to dopaminergic neurons, and depend on the stable association of the ganglioside molecule with the cells. It is suggested that GM1 is not a trophic agent per se, but rather potentiates neurotrophic activities and/or exerts independent influences to which neurons respond only if appropriately supported.
| Original language | English |
|---|---|
| Pages (from-to) | 746-753 |
| Number of pages | 8 |
| Journal | Journal of Neuroscience |
| Volume | 8 |
| Issue number | 3 |
| Publication status | Published - 1988 |
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ASJC Scopus subject areas
- Neuroscience(all)
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Development and survival of neurons in dissociated fetal mesencephalic serum-free cell cultures : II. Modulatory effects of gangliosides. / Leon, A.; Dal Toso, R.; Presti, D.; Benvegnu, D.; Facci, L.; Kirschner, G.; Tettamanti, G.; Toffano, G.
In: Journal of Neuroscience, Vol. 8, No. 3, 1988, p. 746-753.Research output: Contribution to journal › Article
}
TY - JOUR
T1 - Development and survival of neurons in dissociated fetal mesencephalic serum-free cell cultures
T2 - II. Modulatory effects of gangliosides
AU - Leon, A.
AU - Dal Toso, R.
AU - Presti, D.
AU - Benvegnu, D.
AU - Facci, L.
AU - Kirschner, G.
AU - Tettamanti, G.
AU - Toffano, G.
PY - 1988
Y1 - 1988
N2 - This paper analyzes the effects of exogenously supplied GM1 on the development, i.e., specific neurotransmitter uptake capability and survival, of the dopaminergic neurons present in fetal mouse-dissociated mesencephalic cells. Exogenous GM1, but not asialo-GM1, sialic acid, or the oligosaccharide chain of GM1, sialic acid, or the oligosaccharide chain of GM1, enhances in a time- and concentration-dependent manner the specific 3H-dopamine uptake (increase of the apparent V(max) and decrease of the apparent K(m) value) and the long-term survival of the dopaminergic neurons. The GM1 effects on the behavior of the dopaminergic neurons require the presence of cell-derived neuronotrophic influences present within the culture system and are associated with an increase in the response of the cells to the trophic influences. GM1 effects are not limited to dopaminergic neurons, and depend on the stable association of the ganglioside molecule with the cells. It is suggested that GM1 is not a trophic agent per se, but rather potentiates neurotrophic activities and/or exerts independent influences to which neurons respond only if appropriately supported.
AB - This paper analyzes the effects of exogenously supplied GM1 on the development, i.e., specific neurotransmitter uptake capability and survival, of the dopaminergic neurons present in fetal mouse-dissociated mesencephalic cells. Exogenous GM1, but not asialo-GM1, sialic acid, or the oligosaccharide chain of GM1, sialic acid, or the oligosaccharide chain of GM1, enhances in a time- and concentration-dependent manner the specific 3H-dopamine uptake (increase of the apparent V(max) and decrease of the apparent K(m) value) and the long-term survival of the dopaminergic neurons. The GM1 effects on the behavior of the dopaminergic neurons require the presence of cell-derived neuronotrophic influences present within the culture system and are associated with an increase in the response of the cells to the trophic influences. GM1 effects are not limited to dopaminergic neurons, and depend on the stable association of the ganglioside molecule with the cells. It is suggested that GM1 is not a trophic agent per se, but rather potentiates neurotrophic activities and/or exerts independent influences to which neurons respond only if appropriately supported.
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M3 - Article
C2 - 3346718
AN - SCOPUS:0023865447
VL - 8
SP - 746
EP - 753
JO - Journal of Neuroscience
JF - Journal of Neuroscience
SN - 0270-6474
IS - 3
ER -