TY - JOUR
T1 - Development and validation of a HPLC-UV method for the quantification of antiepileptic drugs in dried plasma spots
AU - Baldelli, Sara
AU - Cattaneo, Dario
AU - Giodini, Luciana
AU - Baietto, Lorena
AU - Di Perri, Giovanni
AU - D'Avolio, Antonio
AU - Clementi, Emilio
PY - 2015/2/1
Y1 - 2015/2/1
N2 - Background: Therapeutic drug monitoring (TDM) of antiepileptic drugs is widely used in clinical practice to optimise therapy, but it is limited by technical problems and cost considerations. The aim of the present study was: 1) to validate a chromatographic method for the concomitant determination of levetiracetam, lamotrigine, ethosuximide, felbamate, rufinamide, zonisamide and monohydroxycarbamazepine; 2) to develop it for dried plasma spot (DPS) assessing its reliability against the classical determination from plasma; and 3) test its clinical application. Methods: Extraction of plasma samples and DPS was done by simple precipitation. Chromatographic analysis was performed using high performance liquid chromatography with ultraviolet detection. After validation, both methods were applied for the quantification of plasma samples from patients on antiepileptic therapy. Results: Mean inter-and intra-day accuracy and precision were
AB - Background: Therapeutic drug monitoring (TDM) of antiepileptic drugs is widely used in clinical practice to optimise therapy, but it is limited by technical problems and cost considerations. The aim of the present study was: 1) to validate a chromatographic method for the concomitant determination of levetiracetam, lamotrigine, ethosuximide, felbamate, rufinamide, zonisamide and monohydroxycarbamazepine; 2) to develop it for dried plasma spot (DPS) assessing its reliability against the classical determination from plasma; and 3) test its clinical application. Methods: Extraction of plasma samples and DPS was done by simple precipitation. Chromatographic analysis was performed using high performance liquid chromatography with ultraviolet detection. After validation, both methods were applied for the quantification of plasma samples from patients on antiepileptic therapy. Results: Mean inter-and intra-day accuracy and precision were
KW - antiepileptic drugs
KW - dried plasma spots
KW - high performance liquid chomatography
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U2 - 10.1515/cclm-2014-0472
DO - 10.1515/cclm-2014-0472
M3 - Article
C2 - 25153419
AN - SCOPUS:84923069908
VL - 53
SP - 435
EP - 444
JO - Clinical Chemistry and Laboratory Medicine
JF - Clinical Chemistry and Laboratory Medicine
SN - 1434-6621
IS - 3
ER -