Development and validation of a HPLC-UV method for the quantification of antiepileptic drugs in dried plasma spots

Sara Baldelli, Dario Cattaneo, Luciana Giodini, Lorena Baietto, Giovanni Di Perri, Antonio D'Avolio, Emilio Clementi

Research output: Contribution to journalArticle

Abstract

Background: Therapeutic drug monitoring (TDM) of antiepileptic drugs is widely used in clinical practice to optimise therapy, but it is limited by technical problems and cost considerations. The aim of the present study was: 1) to validate a chromatographic method for the concomitant determination of levetiracetam, lamotrigine, ethosuximide, felbamate, rufinamide, zonisamide and monohydroxycarbamazepine; 2) to develop it for dried plasma spot (DPS) assessing its reliability against the classical determination from plasma; and 3) test its clinical application. Methods: Extraction of plasma samples and DPS was done by simple precipitation. Chromatographic analysis was performed using high performance liquid chromatography with ultraviolet detection. After validation, both methods were applied for the quantification of plasma samples from patients on antiepileptic therapy. Results: Mean inter-and intra-day accuracy and precision were

Original languageEnglish
Pages (from-to)435-444
Number of pages10
JournalClinical Chemistry and Laboratory Medicine
Volume53
Issue number3
DOIs
Publication statusPublished - Feb 1 2015

Keywords

  • antiepileptic drugs
  • dried plasma spots
  • high performance liquid chomatography

ASJC Scopus subject areas

  • Clinical Biochemistry
  • Biochemistry, medical

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