Development and validation of a liquid chromatography-tandem mass spectrometry method for the determination of ST1926, a novel oral antitumor agent, adamantyl retinoid derivative, in plasma of patients in a Phase I study

Federica Sala, Massimo Zucchetti, Renzo Bagnati, Maurizio D'Incalci, Silvia Pace, Francesca Capocasa, Elena Marangon

Research output: Contribution to journalArticle

Abstract

E-3-(4′-Hydroxy-3′-adamantylbiphenyl-4-yl) acrylic acid (ST1926) is a novel oral synthetic adamantyl retinoid derivative, now under early clinical investigation in patients with ovarian cancer. To investigate the pharmacokinetics of this new antitumor agent in human plasma, we developed and validated a high-performance liquid chromatography-tandem mass spectrometry (HPLC-MS/MS) method based on the addition of ST2222 as internal standard and simple protein precipitation with methanol. The method requires a small volume of sample (100 μL); it is rapid and selective, allowing a good resolution of peaks from the plasma matrix in 9 min. The method offers high recovery (>90%), it is sensitive, precise and accurate with overall precision expressed as CV% less than 8.2%. We set the lower limit of quantitation at 20.0 ng/mL and validated the assay up to the concentration of 1000.0 ng/mL. The present method has been successfully applied to study ST1926 pharmacokinetics in patients with advanced ovarian cancer in a Phase I trial, administering the drug orally for five consecutive days. During analysis of the study samples, it became evident the presence of glucuroconjugates of the parent compound, established by LC-Orbitrap MS. Preliminary results show low and variable drug absorption in patients, with extensive glucuroconjugation influencing the bioavailability of ST1926.

Original languageEnglish
Pages (from-to)3118-3126
Number of pages9
JournalJournal of Chromatography B: Analytical Technologies in the Biomedical and Life Sciences
Volume877
Issue number27
DOIs
Publication statusPublished - Oct 1 2009

Keywords

  • Adamantyl retinoid derivative
  • HPLC-MS/MS method development
  • Pharmacokinetics
  • Phase I study
  • ST1926

ASJC Scopus subject areas

  • Biochemistry
  • Analytical Chemistry
  • Cell Biology
  • Clinical Biochemistry

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