Development and validation of a molecular method for the diagnosis of medically important fungal infections

Donata De Marco, Mario Perotti, Cristina M. Ossi, Roberto Burioni, Massimo Clementi, Nicasio Mancini

Research output: Contribution to journalArticlepeer-review

Abstract

The increasing incidence of severe fungal infections highlights the need for rapid and precise identification methods in clinical mycology. The aim of this study was to develop and validate a culture-indipendent molecular approach that could allow the detection of fungal pathogens in clinical samples, with particular attention to the identification of drug-resistant Candida and Aspergillus species. A real-time multiplex PCR assay was developed using TaqMan probes specific for highly discriminating ITS sequences. In its multiplex format the assay showed a high specificity, clearly discriminating among different species, as well as a high sensitivity (20 CFU/1 mL sample), making it a potentially useful starting point for the development of a more complete molecular diagnostic assay.

Original languageEnglish
Pages (from-to)308-312
Number of pages5
JournalNew Microbiologica
Volume30
Issue number3
Publication statusPublished - Jul 2007

Keywords

  • Drug-resistant fungal species
  • Invasive fungal infections
  • Real-time PCR

ASJC Scopus subject areas

  • Microbiology (medical)
  • Microbiology

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