Development and validation of an LC-MS/MS analysis for simultaneous determination of delphinidin-3-glucoside, cyanidin-3-glucoside and cyanidin-3-(6-malonylglucoside) in human plasma and urine after blood orange juice administration

Lucia Giordano, Walter Coletta, Paolo Rapisarda, Maria Benedetta Donati, Domenico Rotilio

Research output: Contribution to journalArticle

Abstract

Blood orange juice has a high content in anthocyanins, especially represented by delphinidin-3-glucoside (D3G), cyanidin-3-glucoside (C3G) and cyanidin-3-(6-malonyl-glucoside) (CMG). An LC-MS/MS method for the simultaneous determination of D3G and C3G in human plasma and urine was developed and validated, After sample preparation by SPE, chromatographic separation was performed with an RP-C18 column, using a water/methanol linear gradient. The quantitation of target compounds was determined by multiple reaction monitoring (MRM) mode, using ESI. The method showed good selectivity, sensitivity (LOD = 0.05 and 0.10 ng/mL for C3G in plasma and urine, respectively; LOD = 0.10 ng/mL for D3G in plasma and urine), linearity (0.20-200 ng/mL; r ≥0.998), intra- and interday precision and accuracy (≥14.6%) and satisfactory recoveries (80.0-110.4%) for the target compounds in both plasma and urine. Stability of analytes in plasma and urine has been investigated in detail. This method was successfully applied to the determination of D3G, C3G and CMG levels in human plasma and urine after the ingestion of a single dose (500 mL) of blood orange juice.

Original languageEnglish
Pages (from-to)3127-3136
Number of pages10
JournalJournal of Separation Science
Volume30
Issue number18
DOIs
Publication statusPublished - Dec 2007

Keywords

  • Blood orange juice
  • Cyanidin-3-(6-malonylglucoside)
  • Cyanidin-3-glucoside
  • Delphinidin-3-glucoside
  • LC-MS/MS

ASJC Scopus subject areas

  • Molecular Medicine
  • Clinical Biochemistry
  • Chemical Engineering(all)
  • Filtration and Separation

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