Abstract
The aim of this study was the development of a method for the determination of 12 urinary monohydroxy metabolites of PAHs, namely 1-hydroxynaphthalene, 2-hydroxynaphthalene, 2-hydroxyfluorene, 9-hydroxyfluorene, 1-hydroxyphenanthrene, 2-hydroxyphenanthrene, 3-hydroxyphenanthrene, 4-hydroxyphenanthrene, 9-hydroxyphenanthrene, 1-hydroxypyrene, 6-hydroxychrysene, and 3-hydroxybenzo[a]pyrene. Analytes were determined in the presence of deuterated analogues as internal standards, by GC/MS operating in the electron impact mode. Sample preparation was performed by enzymatic hydrolysis of glucoronate and sulphate conjugates of hydroxy metabolites of PAHs, liquid-liquid extraction with n-hexane, and derivatization with a silylating reagent. The method is very specific, limits of quantification are in the range 0.1-1.4 μg/l, and range of linearity is from limit of detection to 208 μg/l. Within- and between-run precision, expressed as coefficient of variation, are
| Original language | English |
|---|---|
| Pages (from-to) | 531-540 |
| Number of pages | 10 |
| Journal | Journal of Chromatography B: Analytical Technologies in the Biomedical and Life Sciences |
| Volume | 875 |
| Issue number | 2 |
| DOIs | |
| Publication status | Published - Nov 15 2008 |
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Keywords
- Biological monitoring
- GC/MS
- Polycyclic aromatic hydrocarbons
- Urinary metabolites
ASJC Scopus subject areas
- Biochemistry
- Analytical Chemistry
- Cell Biology
- Clinical Biochemistry
Cite this
Development of a gas chromatography/mass spectrometry method to quantify several urinary monohydroxy metabolites of polycyclic aromatic hydrocarbons in occupationally exposed subjects. / Campo, Laura; Rossella, Federica; Fustinoni, Silvia.
In: Journal of Chromatography B: Analytical Technologies in the Biomedical and Life Sciences, Vol. 875, No. 2, 15.11.2008, p. 531-540.Research output: Contribution to journal › Article
}
TY - JOUR
T1 - Development of a gas chromatography/mass spectrometry method to quantify several urinary monohydroxy metabolites of polycyclic aromatic hydrocarbons in occupationally exposed subjects
AU - Campo, Laura
AU - Rossella, Federica
AU - Fustinoni, Silvia
PY - 2008/11/15
Y1 - 2008/11/15
N2 - The aim of this study was the development of a method for the determination of 12 urinary monohydroxy metabolites of PAHs, namely 1-hydroxynaphthalene, 2-hydroxynaphthalene, 2-hydroxyfluorene, 9-hydroxyfluorene, 1-hydroxyphenanthrene, 2-hydroxyphenanthrene, 3-hydroxyphenanthrene, 4-hydroxyphenanthrene, 9-hydroxyphenanthrene, 1-hydroxypyrene, 6-hydroxychrysene, and 3-hydroxybenzo[a]pyrene. Analytes were determined in the presence of deuterated analogues as internal standards, by GC/MS operating in the electron impact mode. Sample preparation was performed by enzymatic hydrolysis of glucoronate and sulphate conjugates of hydroxy metabolites of PAHs, liquid-liquid extraction with n-hexane, and derivatization with a silylating reagent. The method is very specific, limits of quantification are in the range 0.1-1.4 μg/l, and range of linearity is from limit of detection to 208 μg/l. Within- and between-run precision, expressed as coefficient of variation, are
AB - The aim of this study was the development of a method for the determination of 12 urinary monohydroxy metabolites of PAHs, namely 1-hydroxynaphthalene, 2-hydroxynaphthalene, 2-hydroxyfluorene, 9-hydroxyfluorene, 1-hydroxyphenanthrene, 2-hydroxyphenanthrene, 3-hydroxyphenanthrene, 4-hydroxyphenanthrene, 9-hydroxyphenanthrene, 1-hydroxypyrene, 6-hydroxychrysene, and 3-hydroxybenzo[a]pyrene. Analytes were determined in the presence of deuterated analogues as internal standards, by GC/MS operating in the electron impact mode. Sample preparation was performed by enzymatic hydrolysis of glucoronate and sulphate conjugates of hydroxy metabolites of PAHs, liquid-liquid extraction with n-hexane, and derivatization with a silylating reagent. The method is very specific, limits of quantification are in the range 0.1-1.4 μg/l, and range of linearity is from limit of detection to 208 μg/l. Within- and between-run precision, expressed as coefficient of variation, are
KW - Biological monitoring
KW - GC/MS
KW - Polycyclic aromatic hydrocarbons
KW - Urinary metabolites
UR - http://www.scopus.com/inward/record.url?scp=55349124910&partnerID=8YFLogxK
UR - http://www.scopus.com/inward/citedby.url?scp=55349124910&partnerID=8YFLogxK
U2 - 10.1016/j.jchromb.2008.10.017
DO - 10.1016/j.jchromb.2008.10.017
M3 - Article
C2 - 18952507
AN - SCOPUS:55349124910
VL - 875
SP - 531
EP - 540
JO - Journal of Chromatography B: Analytical Technologies in the Biomedical and Life Sciences
JF - Journal of Chromatography B: Analytical Technologies in the Biomedical and Life Sciences
SN - 1570-0232
IS - 2
ER -