Development of a gas chromatography/mass spectrometry method to quantify several urinary monohydroxy metabolites of polycyclic aromatic hydrocarbons in occupationally exposed subjects

Laura Campo, Federica Rossella, Silvia Fustinoni

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52 Citations (Scopus)

Abstract

The aim of this study was the development of a method for the determination of 12 urinary monohydroxy metabolites of PAHs, namely 1-hydroxynaphthalene, 2-hydroxynaphthalene, 2-hydroxyfluorene, 9-hydroxyfluorene, 1-hydroxyphenanthrene, 2-hydroxyphenanthrene, 3-hydroxyphenanthrene, 4-hydroxyphenanthrene, 9-hydroxyphenanthrene, 1-hydroxypyrene, 6-hydroxychrysene, and 3-hydroxybenzo[a]pyrene. Analytes were determined in the presence of deuterated analogues as internal standards, by GC/MS operating in the electron impact mode. Sample preparation was performed by enzymatic hydrolysis of glucoronate and sulphate conjugates of hydroxy metabolites of PAHs, liquid-liquid extraction with n-hexane, and derivatization with a silylating reagent. The method is very specific, limits of quantification are in the range 0.1-1.4 μg/l, and range of linearity is from limit of detection to 208 μg/l. Within- and between-run precision, expressed as coefficient of variation, are

Original languageEnglish
Pages (from-to)531-540
Number of pages10
JournalJournal of Chromatography B: Analytical Technologies in the Biomedical and Life Sciences
Volume875
Issue number2
DOIs
Publication statusPublished - Nov 15 2008

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Polycyclic Aromatic Hydrocarbons
Polycyclic aromatic hydrocarbons
Metabolites
Gas chromatography
Gas Chromatography-Mass Spectrometry
Mass spectrometry
Liquid-Liquid Extraction
Enzymatic hydrolysis
Liquids
Sulfates
Limit of Detection
Hydrolysis
Electrons
3-hydroxybenzo(a)pyrene
1-hydroxypyrene
9-phenanthrol
2-hydroxyfluorene
1-naphthol
fluoren-9-ol
1-hydroxyphenanthrene

Keywords

  • Biological monitoring
  • GC/MS
  • Polycyclic aromatic hydrocarbons
  • Urinary metabolites

ASJC Scopus subject areas

  • Biochemistry
  • Analytical Chemistry
  • Cell Biology
  • Clinical Biochemistry

Cite this

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N2 - The aim of this study was the development of a method for the determination of 12 urinary monohydroxy metabolites of PAHs, namely 1-hydroxynaphthalene, 2-hydroxynaphthalene, 2-hydroxyfluorene, 9-hydroxyfluorene, 1-hydroxyphenanthrene, 2-hydroxyphenanthrene, 3-hydroxyphenanthrene, 4-hydroxyphenanthrene, 9-hydroxyphenanthrene, 1-hydroxypyrene, 6-hydroxychrysene, and 3-hydroxybenzo[a]pyrene. Analytes were determined in the presence of deuterated analogues as internal standards, by GC/MS operating in the electron impact mode. Sample preparation was performed by enzymatic hydrolysis of glucoronate and sulphate conjugates of hydroxy metabolites of PAHs, liquid-liquid extraction with n-hexane, and derivatization with a silylating reagent. The method is very specific, limits of quantification are in the range 0.1-1.4 μg/l, and range of linearity is from limit of detection to 208 μg/l. Within- and between-run precision, expressed as coefficient of variation, are

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