Development of a mass spectrometric method to quantitate platelet activating factor in mouse urine

E. Benfenati, D. Macconi, M. Noris, G. Icardi, L. Bettazzoli, G. De Bellis, M. Gavinelli, S. Rotondo, G. Remuzzzi

Research output: Contribution to journalArticle

Abstract

Platelet activating factor (PAF) is a lipid mediator of inflammation released by a variety of stimulated inflammatory cells. It may be involved in immune glomerulonephritis. Thus, its measurement in urine could give information on the mechanism of this disease. We present here a method to measure PAF in mouse urine, using gas-liquid chromatography-mass spectrometry (GLC-MS) in the selected ion recording (SIR) mode. Before instrumental analysis, the extracted and purified samples were hydrolyzed and derivatized with pentafluorobenzoyl chloride. Different experimental conditions are presented and discussed to corroborate the analytic findings. PAF levels in mouse urine were 2.08 ± 0.46 ng/24 h. This procedure might represent a new experimental tool to establish the possible role of PAF as mediator of tissue damage in renal disease.

Original languageEnglish
Pages (from-to)1977-1981
Number of pages5
JournalJournal of Lipid Research
Volume30
Issue number12
Publication statusPublished - 1989

Keywords

  • selected ion recording

ASJC Scopus subject areas

  • Endocrinology

Fingerprint Dive into the research topics of 'Development of a mass spectrometric method to quantitate platelet activating factor in mouse urine'. Together they form a unique fingerprint.

  • Cite this

    Benfenati, E., Macconi, D., Noris, M., Icardi, G., Bettazzoli, L., De Bellis, G., Gavinelli, M., Rotondo, S., & Remuzzzi, G. (1989). Development of a mass spectrometric method to quantitate platelet activating factor in mouse urine. Journal of Lipid Research, 30(12), 1977-1981.