Development of a multigenic plasmid vector for HCV DNA immunization

S. Papa, M. Rinaldi, A. Mangia, P. Parrella, E. Signori, L. Lombardi, V. M. Fazio

Research output: Contribution to journalArticlepeer-review

Abstract

HCV viral nucleocapsid protein (C), non-structural protein 3 (NS3) and the envelope glycoproteins E1 and E2 are candidate immune targets for developing anti-HCV DNA vaccine. Nevertheless, the immune response elicited by these antigens often appears weak and/or transient. Different approaches have been studied for enhancing and/or modulating the immune response of the DNA vaccine. On the basis of a prototype multigenic plasmid vector constituted of two different transcription cassettes (pRC100), we have developed a plasmid vector that allows the independent and simultaneous expression of murine IL2 and of an antigenic domain of the HCV NS3 C terminus (pRC112-HCV). The highly conserved NS3 region spans from nt 4403 to nt 4829 and contains two putative B and T epitopes. The development of this multigenic plasmid vector may combine the expression and local production of an immunomodulatory molecule (mlL2) together with the possibility of addressing the host immune response to the most immunogenic and conserved epitopes, specifically tailored in the plasmid vector.

Original languageEnglish
Pages (from-to)315-319
Number of pages5
JournalResearch in Virology
Volume149
Issue number5
DOIs
Publication statusPublished - Sep 1998

Keywords

  • B/T epitopes
  • DNA vaccination
  • HCV NS3
  • Multigenic plasmid vector
  • Murine lL2

ASJC Scopus subject areas

  • Virology
  • Immunology

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