Development of a new tool for 3D modeling for regenerative medicine

Sabata Martino, Filippo Mattoli, Roberto Tiribuzi, Francesco D'Angelo, Ilaria Di Girolamo, Mattia Quattrocelli, Simona Montesano, Lucia Crispoltoni, Vasileios Oikonomou, Maria Gabriella Cusella De Angelis, Peggy Marconi, Antonio Orlacchio, Maurilio Sampaolesi, Aldo Orlacchio

Research output: Contribution to journalArticle

Abstract

The effectiveness of therapeutic treatment based on regenerative medicine for degenerative diseases (i.e., neurodegenerative or cardiac diseases) requires tools allowing the visualization and analysis of the three-dimensional (3D) distribution of target drugs within the tissue. Here, we present a new computational procedure able to overcome the limitations of visual analysis emerging by the examination of a molecular signal within images of serial tissue/organ sections by using the conventional techniques. Together with the 3D anatomical reconstitution of the tissue/organ, our framework allows the detection of signals of different origins (e.g., marked generic molecules, colorimetric, or fluorimetric substrates for enzymes; microRNA; recombinant protein). Remarkably, the application does not require the employment of specific tracking reagents for the imaging analysis. We report two different representative applications: the first shows the reconstruction of a 3D model of mouse brain with the analysis of the distribution of the -Galactosidase, the second shows the reconstruction of a 3D mouse heart with the measurement of the cardiac volume.

Original languageEnglish
Article number236854
JournalInternational Journal of Biomedical Imaging
Volume2011
DOIs
Publication statusPublished - 2011

ASJC Scopus subject areas

  • Radiology Nuclear Medicine and imaging

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  • Cite this

    Martino, S., Mattoli, F., Tiribuzi, R., D'Angelo, F., Di Girolamo, I., Quattrocelli, M., Montesano, S., Crispoltoni, L., Oikonomou, V., Cusella De Angelis, M. G., Marconi, P., Orlacchio, A., Sampaolesi, M., & Orlacchio, A. (2011). Development of a new tool for 3D modeling for regenerative medicine. International Journal of Biomedical Imaging, 2011, [236854]. https://doi.org/10.1155/2011/236854