TY - JOUR
T1 - Development of a patient-derived xenograft (PDX) of breast cancer bone metastasis in a Zebrafish model
AU - Mercatali, Laura
AU - La Manna, Federico
AU - Groenewoud, Arwin
AU - Casadei, Roberto
AU - Recine, Federica
AU - Miserocchi, Giacomo
AU - Pieri, F.
AU - Liverani, Chiara
AU - Bongiovanni, Alberto
AU - Spadazzi, Chiara
AU - De Vita, Alessandro
AU - van der Pluijm, Gabri
AU - Giorgini, A.
AU - Biagini, Roberto
AU - Amadori, Dino
AU - Ibrahim, Toni
AU - Snaar-Jagalska, Ewa
PY - 2016/8/22
Y1 - 2016/8/22
N2 - Bone metastasis is a complex process that needs to be better understood in order to help clinicians prevent and treat it. Xenografts using patient-derived material (PDX) rather than cancer cell lines are a novel approach that guarantees more clinically realistic results. A primary culture of bone metastasis derived from a 67-year-old patient with breast cancer was cultured and then injected into zebrafish (ZF) embryos to study its metastatic potential. In vivo behavior and results of gene expression analyses of the primary culture were compared with those of cancer cell lines with different metastatic potential (MCF7 and MDA-MB-231). The MCF7 cell line, which has the same hormonal receptor status as the bone metastasis primary culture, did not survive in the in vivo model. Conversely, MDA-MB-231 disseminated and colonized different parts of the ZF, including caudal hematopoietic tissues (CHT), revealing a migratory phenotype. Primary culture cells disseminated and in later stages extravasated from the vessels, engrafting into ZF tissues and reaching the CHT. Primary cell behavior reflected the clinical course of the patient’s medical history. Our results underline the potential for using PDX models in bone metastasis research and outline new methods for the clinical application of this in vivo model.
AB - Bone metastasis is a complex process that needs to be better understood in order to help clinicians prevent and treat it. Xenografts using patient-derived material (PDX) rather than cancer cell lines are a novel approach that guarantees more clinically realistic results. A primary culture of bone metastasis derived from a 67-year-old patient with breast cancer was cultured and then injected into zebrafish (ZF) embryos to study its metastatic potential. In vivo behavior and results of gene expression analyses of the primary culture were compared with those of cancer cell lines with different metastatic potential (MCF7 and MDA-MB-231). The MCF7 cell line, which has the same hormonal receptor status as the bone metastasis primary culture, did not survive in the in vivo model. Conversely, MDA-MB-231 disseminated and colonized different parts of the ZF, including caudal hematopoietic tissues (CHT), revealing a migratory phenotype. Primary culture cells disseminated and in later stages extravasated from the vessels, engrafting into ZF tissues and reaching the CHT. Primary cell behavior reflected the clinical course of the patient’s medical history. Our results underline the potential for using PDX models in bone metastasis research and outline new methods for the clinical application of this in vivo model.
KW - Bone metastasis
KW - Breast cancer
KW - Patient-derived xenograft
KW - Zebrafish model
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U2 - 10.3390/ijms17081375
DO - 10.3390/ijms17081375
M3 - Article
VL - 17
JO - International Journal of Molecular Sciences
JF - International Journal of Molecular Sciences
SN - 1661-6596
IS - 8
M1 - 1375
ER -