Development of a polymerase chain reaction and restriction typing assay for the diagnosis of bovine herpesvirus 1, bovine herpesvirus 2, and bovine herpesvirus 4 infections

Luciana De-Giuli, Simone Magnino, Pier Giorgio Vigo, Iris Labalestra, Massimo Fabbi

Research output: Contribution to journalArticle

Abstract

A multiplex polymerase chain reaction (PCR) method coupled with a restriction analysis of PCR products (PCR with restriction fragment length polymorphism) was developed for the simultaneous detection of bovine herpesvirus 1, bovine herpesvirus 2, and bovine herpesvirus 4 infections. The specificity, sensitivity, and practical diagnostic applicability of this method were evaluated. This assay may be also adapted to the diagnosis of suid herpesvirus 1 and equine herpesviruses 1 and 3 and could become a powerful diagnostic tool.

Original languageEnglish
Pages (from-to)353-356
Number of pages4
JournalJournal of Veterinary Diagnostic Investigation
Volume14
Issue number4
Publication statusPublished - Jul 2002

ASJC Scopus subject areas

  • Microbiology
  • veterinary(all)

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