Abstract
A multiplex polymerase chain reaction (PCR) method coupled with a restriction analysis of PCR products (PCR with restriction fragment length polymorphism) was developed for the simultaneous detection of bovine herpesvirus 1, bovine herpesvirus 2, and bovine herpesvirus 4 infections. The specificity, sensitivity, and practical diagnostic applicability of this method were evaluated. This assay may be also adapted to the diagnosis of suid herpesvirus 1 and equine herpesviruses 1 and 3 and could become a powerful diagnostic tool.
| Original language | English |
|---|---|
| Pages (from-to) | 353-356 |
| Number of pages | 4 |
| Journal | Journal of Veterinary Diagnostic Investigation |
| Volume | 14 |
| Issue number | 4 |
| Publication status | Published - Jul 2002 |
ASJC Scopus subject areas
- Microbiology
- veterinary(all)
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De-Giuli, L., Magnino, S., Vigo, P. G., Labalestra, I., & Fabbi, M. (2002). Development of a polymerase chain reaction and restriction typing assay for the diagnosis of bovine herpesvirus 1, bovine herpesvirus 2, and bovine herpesvirus 4 infections. Journal of Veterinary Diagnostic Investigation, 14(4), 353-356.