Abstract
A multiplex polymerase chain reaction (PCR) method coupled with a restriction analysis of PCR products (PCR with restriction fragment length polymorphism) was developed for the simultaneous detection of bovine herpesvirus 1, bovine herpesvirus 2, and bovine herpesvirus 4 infections. The specificity, sensitivity, and practical diagnostic applicability of this method were evaluated. This assay may be also adapted to the diagnosis of suid herpesvirus 1 and equine herpesviruses 1 and 3 and could become a powerful diagnostic tool.
| Original language | English |
|---|---|
| Pages (from-to) | 353-356 |
| Number of pages | 4 |
| Journal | Journal of Veterinary Diagnostic Investigation |
| Volume | 14 |
| Issue number | 4 |
| Publication status | Published - Jul 2002 |
Fingerprint
ASJC Scopus subject areas
- Microbiology
- veterinary(all)
Cite this
Development of a polymerase chain reaction and restriction typing assay for the diagnosis of bovine herpesvirus 1, bovine herpesvirus 2, and bovine herpesvirus 4 infections. / De-Giuli, Luciana; Magnino, Simone; Vigo, Pier Giorgio; Labalestra, Iris; Fabbi, Massimo.
In: Journal of Veterinary Diagnostic Investigation, Vol. 14, No. 4, 07.2002, p. 353-356.Research output: Contribution to journal › Article
}
TY - JOUR
T1 - Development of a polymerase chain reaction and restriction typing assay for the diagnosis of bovine herpesvirus 1, bovine herpesvirus 2, and bovine herpesvirus 4 infections
AU - De-Giuli, Luciana
AU - Magnino, Simone
AU - Vigo, Pier Giorgio
AU - Labalestra, Iris
AU - Fabbi, Massimo
PY - 2002/7
Y1 - 2002/7
N2 - A multiplex polymerase chain reaction (PCR) method coupled with a restriction analysis of PCR products (PCR with restriction fragment length polymorphism) was developed for the simultaneous detection of bovine herpesvirus 1, bovine herpesvirus 2, and bovine herpesvirus 4 infections. The specificity, sensitivity, and practical diagnostic applicability of this method were evaluated. This assay may be also adapted to the diagnosis of suid herpesvirus 1 and equine herpesviruses 1 and 3 and could become a powerful diagnostic tool.
AB - A multiplex polymerase chain reaction (PCR) method coupled with a restriction analysis of PCR products (PCR with restriction fragment length polymorphism) was developed for the simultaneous detection of bovine herpesvirus 1, bovine herpesvirus 2, and bovine herpesvirus 4 infections. The specificity, sensitivity, and practical diagnostic applicability of this method were evaluated. This assay may be also adapted to the diagnosis of suid herpesvirus 1 and equine herpesviruses 1 and 3 and could become a powerful diagnostic tool.
UR - http://www.scopus.com/inward/record.url?scp=0036652897&partnerID=8YFLogxK
UR - http://www.scopus.com/inward/citedby.url?scp=0036652897&partnerID=8YFLogxK
M3 - Article
C2 - 12152821
AN - SCOPUS:0036652897
VL - 14
SP - 353
EP - 356
JO - Journal of Veterinary Diagnostic Investigation
JF - Journal of Veterinary Diagnostic Investigation
SN - 1040-6387
IS - 4
ER -