Development of a polymerase chain reaction and restriction typing assay for the diagnosis of bovine herpesvirus 1, bovine herpesvirus 2, and bovine herpesvirus 4 infections

Luciana De-Giuli, Simone Magnino, Pier Giorgio Vigo, Iris Labalestra, Massimo Fabbi

Research output: Contribution to journalArticle

7 Citations (Scopus)

Abstract

A multiplex polymerase chain reaction (PCR) method coupled with a restriction analysis of PCR products (PCR with restriction fragment length polymorphism) was developed for the simultaneous detection of bovine herpesvirus 1, bovine herpesvirus 2, and bovine herpesvirus 4 infections. The specificity, sensitivity, and practical diagnostic applicability of this method were evaluated. This assay may be also adapted to the diagnosis of suid herpesvirus 1 and equine herpesviruses 1 and 3 and could become a powerful diagnostic tool.

Original languageEnglish
Pages (from-to)353-356
Number of pages4
JournalJournal of Veterinary Diagnostic Investigation
Volume14
Issue number4
Publication statusPublished - Jul 2002

Fingerprint

Bovine Herpesvirus 2
Bovine Herpesvirus 4
Bovine herpesvirus 2
Bovine herpesvirus 4
Bovine Herpesvirus 1
Herpesviridae Infections
Bovine herpesvirus 1
Equid Herpesvirus 3
polymerase chain reaction
Equid Herpesvirus 1
Suid Herpesvirus 1
Polymerase Chain Reaction
Equid herpesvirus 3
Multiplex Polymerase Chain Reaction
assays
Restriction Fragment Length Polymorphisms
infection
Equid herpesvirus 1
Suid herpesvirus 1
Sensitivity and Specificity

ASJC Scopus subject areas

  • Microbiology
  • veterinary(all)

Cite this

Development of a polymerase chain reaction and restriction typing assay for the diagnosis of bovine herpesvirus 1, bovine herpesvirus 2, and bovine herpesvirus 4 infections. / De-Giuli, Luciana; Magnino, Simone; Vigo, Pier Giorgio; Labalestra, Iris; Fabbi, Massimo.

In: Journal of Veterinary Diagnostic Investigation, Vol. 14, No. 4, 07.2002, p. 353-356.

Research output: Contribution to journalArticle

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