Development of a rapid and ultrasensitive RIA method for estrogen (E2, E1, E1-S) determination with selective solid phase extraction

A. Martinetti, E. Seregni, E. Bajetta, G. F. Bolelli, L. Ferrari, S. Massaron, C. Botti, E. Bombardieri

Research output: Contribution to journalArticle

Abstract

The inhibition of the proliferative stimulation exercised by estrogens on neoplastic cells is the goal of all endocrine therapies in breast cancer. Under various circumstances, e.g. with the use of aromatase inhibitors, this result can be obtained by blocking the synthetic pathway and, conseguently, by lowering the circulating levels of estradiol (E2), estrone (E1), and estrone sulfate (E1-S). The evaluation of these hormones in plasma could therefore represent a useful indicator of the biological efficacy of the therapy. However, the measurement of circulating steroids in a large series of patients is often a complicated procedure. Indirect methods of extraction are time consuming and expensive while the analytical sensitivity of direct methods is not sufficient to measure the residual levels ofE2, E1, and E1-S. In this paper we describe a novel extraction method for the evaluation of plasma levels of E2, E1, and E1-S. This new method consists of solid phase extraction followed by a highly specific radioimmunoassay. The sensitivity of the assay is 0.6 pg/ml, 2.0 pg/ml and 7.0 pg/ml for E2, El, and E1-S, respectively.

Original languageEnglish
Pages (from-to)102-105
Number of pages4
JournalInternational Journal of Biological Markers
Volume12
Issue number3
Publication statusPublished - Jul 1997

Keywords

  • Assay
  • Estradiol
  • Estrone
  • Estrone sulfate
  • Hormonal treatmtent

ASJC Scopus subject areas

  • Immunology
  • Biochemistry

Fingerprint Dive into the research topics of 'Development of a rapid and ultrasensitive RIA method for estrogen (E2, E1, E1-S) determination with selective solid phase extraction'. Together they form a unique fingerprint.

  • Cite this