TY - JOUR
T1 - Development of a Sensing Array for Human Breath Analysis Based on SWCNT Layers Functionalized with Semiconductor Organic Molecules
AU - Freddi, Sonia
AU - Emelianov, Aleksei V.
AU - Bobrinetskiy, Ivan I.
AU - Drera, Giovanni
AU - Pagliara, Stefania
AU - Kopylova, Daria S.
AU - Chiesa, Maria
AU - Santini, Giuseppe
AU - Mores, Nadia
AU - Moscato, Umberto
AU - Nasibulin, Albert G.
AU - Montuschi, Paolo
AU - Sangaletti, Luigi
N1 - Funding Information:
L.S. and P.M. share the senior authorship of this article. L.S. and P.M. share the corresponding authorship of this article. S.F., S.P., G.D., M.C., G.S., N.M., U.M., P.M., and L.S. acknowledge support from ANAPNOI project, funded by Catholic University of the Sacred Heart under the D3.2 Program, and Marco della Vedova for advice on the use of the statistical analysis R package. A.V.E., I.I.B., D.S.K., and A.G.N. acknowledge the Russian Science Foundation (Project No. 19-19-00401, fabrication of functionalized CNT sensors, and No. 17-19-01787, synthesis of carbon nanotubes). The authors would like to acknowledge Dr. S.S. Abramchuk for his assistance in TEM experiments.
Publisher Copyright:
© 2020 WILEY-VCH Verlag GmbH & Co. KGaA, Weinheim
Copyright:
Copyright 2020 Elsevier B.V., All rights reserved.
PY - 2020/6/1
Y1 - 2020/6/1
N2 - A sensor array based on heterojunctions between semiconducting organic layers and single walled carbon nanotube (SWCNT) films is produced to explore applications in breathomics, the molecular analysis of exhaled breath. The array is exposed to gas/volatiles relevant to specific diseases (ammonia, ethanol, acetone, 2-propanol, sodium hypochlorite, benzene, hydrogen sulfide, and nitrogen dioxide). Then, to evaluate its capability to operate with real relevant biological samples the array is exposed to human breath exhaled from healthy subjects. Finally, to provide a proof of concept of its diagnostic potential, the array is exposed to exhaled breath samples collected from subjects with chronic obstructive pulmonary disease (COPD), an airway chronic inflammatory disease not yet investigated with CNT-based sensor arrays, and breathprints are compared with those obtained from of healthy subjects. Principal component analysis shows that the sensor array is able to detect various target gas/volatiles with a clear fingerprint on a 2D subspace, is suitable for breath profiling in exhaled human breath, and is able to distinguish subjects with COPD from healthy subjects based on their breathprints. This classification ability is further improved by selecting the most responsive sensors to nitrogen dioxide, a potential biomarker of COPD.
AB - A sensor array based on heterojunctions between semiconducting organic layers and single walled carbon nanotube (SWCNT) films is produced to explore applications in breathomics, the molecular analysis of exhaled breath. The array is exposed to gas/volatiles relevant to specific diseases (ammonia, ethanol, acetone, 2-propanol, sodium hypochlorite, benzene, hydrogen sulfide, and nitrogen dioxide). Then, to evaluate its capability to operate with real relevant biological samples the array is exposed to human breath exhaled from healthy subjects. Finally, to provide a proof of concept of its diagnostic potential, the array is exposed to exhaled breath samples collected from subjects with chronic obstructive pulmonary disease (COPD), an airway chronic inflammatory disease not yet investigated with CNT-based sensor arrays, and breathprints are compared with those obtained from of healthy subjects. Principal component analysis shows that the sensor array is able to detect various target gas/volatiles with a clear fingerprint on a 2D subspace, is suitable for breath profiling in exhaled human breath, and is able to distinguish subjects with COPD from healthy subjects based on their breathprints. This classification ability is further improved by selecting the most responsive sensors to nitrogen dioxide, a potential biomarker of COPD.
KW - breathomics
KW - carbon nanotubes
KW - chronic obstructive pulmonary disease
KW - electronic noses
KW - hybrid heterojunctions
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U2 - 10.1002/adhm.202000377
DO - 10.1002/adhm.202000377
M3 - Article
C2 - 32378358
AN - SCOPUS:85085116129
VL - 9
JO - Advanced healthcare materials
JF - Advanced healthcare materials
SN - 2192-2640
IS - 12
M1 - 2000377
ER -