Development of a stable insect cell line constitutively expressing rotavirus VP2

Zabihollah Shoja, Maria Tagliamonte, Somayeh Jalilvand, Talat Mokhtari-Azad, Rasool Hamkar, Shohreh Shahmahmoodi, Farhad Rezaei, MariaLina Tornesello, Franco M. Buonaguro, Luigi Buonaguro, Rakhshandeh Nategh

Research output: Contribution to journalArticle

Abstract

An insect High-Five cell line was generated constitutively and stably expressing the VP2 protein of rotavirus RF strain leading to the formation of core-like particles. The integration of the VP2 gene was confirmed by PCR, Real-time PCR and Southern blot, and the protein expression was confirmed by Western blot and immunofluorescence microscopy. Integrity and self assembly of VP2 as core-like particles was demonstrated by electron microscopy. The High-Five cell system stably expressing rotavirus core-like particles can be applied to the study of viral protein structure and functions; it may be useful for vaccine development, gene therapy and drug delivery.

Original languageEnglish
Pages (from-to)66-74
Number of pages9
JournalVirus Research
Volume172
Issue number1-2
DOIs
Publication statusPublished - Mar 2013

Fingerprint

Viral Structures
Rotavirus
Viral Proteins
Southern Blotting
Fluorescence Microscopy
Genetic Therapy
Insects
Real-Time Polymerase Chain Reaction
Electron Microscopy
Vaccines
Western Blotting
Cell Line
Polymerase Chain Reaction
Pharmaceutical Preparations
Genes
Proteins
Rotavirus VP2 protein

Keywords

  • Core-like particles
  • High-Five cell
  • Rotavirus
  • Self assembly
  • VP2

ASJC Scopus subject areas

  • Virology
  • Infectious Diseases
  • Cancer Research

Cite this

Shoja, Z., Tagliamonte, M., Jalilvand, S., Mokhtari-Azad, T., Hamkar, R., Shahmahmoodi, S., ... Nategh, R. (2013). Development of a stable insect cell line constitutively expressing rotavirus VP2. Virus Research, 172(1-2), 66-74. https://doi.org/10.1016/j.virusres.2012.12.015

Development of a stable insect cell line constitutively expressing rotavirus VP2. / Shoja, Zabihollah; Tagliamonte, Maria; Jalilvand, Somayeh; Mokhtari-Azad, Talat; Hamkar, Rasool; Shahmahmoodi, Shohreh; Rezaei, Farhad; Tornesello, MariaLina; Buonaguro, Franco M.; Buonaguro, Luigi; Nategh, Rakhshandeh.

In: Virus Research, Vol. 172, No. 1-2, 03.2013, p. 66-74.

Research output: Contribution to journalArticle

Shoja, Z, Tagliamonte, M, Jalilvand, S, Mokhtari-Azad, T, Hamkar, R, Shahmahmoodi, S, Rezaei, F, Tornesello, M, Buonaguro, FM, Buonaguro, L & Nategh, R 2013, 'Development of a stable insect cell line constitutively expressing rotavirus VP2', Virus Research, vol. 172, no. 1-2, pp. 66-74. https://doi.org/10.1016/j.virusres.2012.12.015
Shoja Z, Tagliamonte M, Jalilvand S, Mokhtari-Azad T, Hamkar R, Shahmahmoodi S et al. Development of a stable insect cell line constitutively expressing rotavirus VP2. Virus Research. 2013 Mar;172(1-2):66-74. https://doi.org/10.1016/j.virusres.2012.12.015
Shoja, Zabihollah ; Tagliamonte, Maria ; Jalilvand, Somayeh ; Mokhtari-Azad, Talat ; Hamkar, Rasool ; Shahmahmoodi, Shohreh ; Rezaei, Farhad ; Tornesello, MariaLina ; Buonaguro, Franco M. ; Buonaguro, Luigi ; Nategh, Rakhshandeh. / Development of a stable insect cell line constitutively expressing rotavirus VP2. In: Virus Research. 2013 ; Vol. 172, No. 1-2. pp. 66-74.
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