Development of a stable insect cell line constitutively expressing rotavirus VP2

Zabihollah Shoja; Maria Tagliamonte; Somayeh Jalilvand; Talat Mokhtari-Azad; Rasool Hamkar; Shohreh Shahmahmoodi; Farhad Rezaei; MariaLina Tornesello; Franco M. Buonaguro; Luigi Buonaguro; Rakhshandeh Nategh

doi:10.1016/j.virusres.2012.12.015

Development of a stable insect cell line constitutively expressing rotavirus VP2

Zabihollah Shoja, Maria Tagliamonte, Somayeh Jalilvand, Talat Mokhtari-Azad, Rasool Hamkar, Shohreh Shahmahmoodi, Farhad Rezaei, MariaLina Tornesello, Franco M. Buonaguro, Luigi Buonaguro, Rakhshandeh Nategh

Istituto Nazionale Tumori Fondazione G. Pascale

Research output: Contribution to journal › Article › peer-review

Abstract

An insect High-Five cell line was generated constitutively and stably expressing the VP2 protein of rotavirus RF strain leading to the formation of core-like particles. The integration of the VP2 gene was confirmed by PCR, Real-time PCR and Southern blot, and the protein expression was confirmed by Western blot and immunofluorescence microscopy. Integrity and self assembly of VP2 as core-like particles was demonstrated by electron microscopy. The High-Five cell system stably expressing rotavirus core-like particles can be applied to the study of viral protein structure and functions; it may be useful for vaccine development, gene therapy and drug delivery.

Original language	English
Pages (from-to)	66-74
Number of pages	9
Journal	Virus Research
Volume	172
Issue number	1-2
DOIs	https://doi.org/10.1016/j.virusres.2012.12.015
Publication status	Published - Mar 2013

Keywords

Core-like particles
High-Five cell
Rotavirus
Self assembly
VP2

ASJC Scopus subject areas

Virology
Infectious Diseases
Cancer Research

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Development of a stable insect cell line constitutively expressing rotavirus VP2. / Shoja, Zabihollah; Tagliamonte, Maria; Jalilvand, Somayeh; Mokhtari-Azad, Talat; Hamkar, Rasool; Shahmahmoodi, Shohreh; Rezaei, Farhad; Tornesello, MariaLina ; Buonaguro, Franco M.; Buonaguro, Luigi; Nategh, Rakhshandeh.

In: Virus Research, Vol. 172, No. 1-2, 03.2013, p. 66-74.

Research output: Contribution to journal › Article › peer-review

Shoja, Zabihollah ; Tagliamonte, Maria ; Jalilvand, Somayeh ; Mokhtari-Azad, Talat ; Hamkar, Rasool ; Shahmahmoodi, Shohreh ; Rezaei, Farhad ; Tornesello, MariaLina ; Buonaguro, Franco M. ; Buonaguro, Luigi ; Nategh, Rakhshandeh. / Development of a stable insect cell line constitutively expressing rotavirus VP2. In: Virus Research. 2013 ; Vol. 172, No. 1-2. pp. 66-74.

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abstract = "An insect High-Five cell line was generated constitutively and stably expressing the VP2 protein of rotavirus RF strain leading to the formation of core-like particles. The integration of the VP2 gene was confirmed by PCR, Real-time PCR and Southern blot, and the protein expression was confirmed by Western blot and immunofluorescence microscopy. Integrity and self assembly of VP2 as core-like particles was demonstrated by electron microscopy. The High-Five cell system stably expressing rotavirus core-like particles can be applied to the study of viral protein structure and functions; it may be useful for vaccine development, gene therapy and drug delivery.",

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