Development of a stable insect cell line constitutively expressing rotavirus VP2

Zabihollah Shoja, Maria Tagliamonte, Somayeh Jalilvand, Talat Mokhtari-Azad, Rasool Hamkar, Shohreh Shahmahmoodi, Farhad Rezaei, MariaLina Tornesello, Franco M. Buonaguro, Luigi Buonaguro, Rakhshandeh Nategh

Research output: Contribution to journalArticlepeer-review

Abstract

An insect High-Five cell line was generated constitutively and stably expressing the VP2 protein of rotavirus RF strain leading to the formation of core-like particles. The integration of the VP2 gene was confirmed by PCR, Real-time PCR and Southern blot, and the protein expression was confirmed by Western blot and immunofluorescence microscopy. Integrity and self assembly of VP2 as core-like particles was demonstrated by electron microscopy. The High-Five cell system stably expressing rotavirus core-like particles can be applied to the study of viral protein structure and functions; it may be useful for vaccine development, gene therapy and drug delivery.

Original languageEnglish
Pages (from-to)66-74
Number of pages9
JournalVirus Research
Volume172
Issue number1-2
DOIs
Publication statusPublished - Mar 2013

Keywords

  • Core-like particles
  • High-Five cell
  • Rotavirus
  • Self assembly
  • VP2

ASJC Scopus subject areas

  • Virology
  • Infectious Diseases
  • Cancer Research

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