Abstract
An insect High-Five cell line was generated constitutively and stably expressing the VP2 protein of rotavirus RF strain leading to the formation of core-like particles. The integration of the VP2 gene was confirmed by PCR, Real-time PCR and Southern blot, and the protein expression was confirmed by Western blot and immunofluorescence microscopy. Integrity and self assembly of VP2 as core-like particles was demonstrated by electron microscopy. The High-Five cell system stably expressing rotavirus core-like particles can be applied to the study of viral protein structure and functions; it may be useful for vaccine development, gene therapy and drug delivery.
| Original language | English |
|---|---|
| Pages (from-to) | 66-74 |
| Number of pages | 9 |
| Journal | Virus Research |
| Volume | 172 |
| Issue number | 1-2 |
| DOIs | |
| Publication status | Published - Mar 2013 |
Fingerprint
Keywords
- Core-like particles
- High-Five cell
- Rotavirus
- Self assembly
- VP2
ASJC Scopus subject areas
- Virology
- Infectious Diseases
- Cancer Research
Cite this
Development of a stable insect cell line constitutively expressing rotavirus VP2. / Shoja, Zabihollah; Tagliamonte, Maria; Jalilvand, Somayeh; Mokhtari-Azad, Talat; Hamkar, Rasool; Shahmahmoodi, Shohreh; Rezaei, Farhad; Tornesello, MariaLina; Buonaguro, Franco M.; Buonaguro, Luigi; Nategh, Rakhshandeh.
In: Virus Research, Vol. 172, No. 1-2, 03.2013, p. 66-74.Research output: Contribution to journal › Article
}
TY - JOUR
T1 - Development of a stable insect cell line constitutively expressing rotavirus VP2
AU - Shoja, Zabihollah
AU - Tagliamonte, Maria
AU - Jalilvand, Somayeh
AU - Mokhtari-Azad, Talat
AU - Hamkar, Rasool
AU - Shahmahmoodi, Shohreh
AU - Rezaei, Farhad
AU - Tornesello, MariaLina
AU - Buonaguro, Franco M.
AU - Buonaguro, Luigi
AU - Nategh, Rakhshandeh
PY - 2013/3
Y1 - 2013/3
N2 - An insect High-Five cell line was generated constitutively and stably expressing the VP2 protein of rotavirus RF strain leading to the formation of core-like particles. The integration of the VP2 gene was confirmed by PCR, Real-time PCR and Southern blot, and the protein expression was confirmed by Western blot and immunofluorescence microscopy. Integrity and self assembly of VP2 as core-like particles was demonstrated by electron microscopy. The High-Five cell system stably expressing rotavirus core-like particles can be applied to the study of viral protein structure and functions; it may be useful for vaccine development, gene therapy and drug delivery.
AB - An insect High-Five cell line was generated constitutively and stably expressing the VP2 protein of rotavirus RF strain leading to the formation of core-like particles. The integration of the VP2 gene was confirmed by PCR, Real-time PCR and Southern blot, and the protein expression was confirmed by Western blot and immunofluorescence microscopy. Integrity and self assembly of VP2 as core-like particles was demonstrated by electron microscopy. The High-Five cell system stably expressing rotavirus core-like particles can be applied to the study of viral protein structure and functions; it may be useful for vaccine development, gene therapy and drug delivery.
KW - Core-like particles
KW - High-Five cell
KW - Rotavirus
KW - Self assembly
KW - VP2
UR - http://www.scopus.com/inward/record.url?scp=84873750109&partnerID=8YFLogxK
UR - http://www.scopus.com/inward/citedby.url?scp=84873750109&partnerID=8YFLogxK
U2 - 10.1016/j.virusres.2012.12.015
DO - 10.1016/j.virusres.2012.12.015
M3 - Article
C2 - 23287060
AN - SCOPUS:84873750109
VL - 172
SP - 66
EP - 74
JO - Virus Research
JF - Virus Research
SN - 0168-1702
IS - 1-2
ER -