Development of an immunoassay for the derived-peptide of chromogranin A, Vasostatin-I (1-76)

Assessment of severity in patients with sepsis

Hélène Chung, Angelo Corti, Luca Crippa, Francis Schneider, Marie Hélène Metz-Boutigue, Patrick Garnero

Research output: Contribution to journalArticle

8 Citations (Scopus)

Abstract

Context: Proteolytic fragments of chromogranin A (CgA) including the CgA 1-76 fragment (called vasostatin-I [VS-I]) could be a useful biomarker of sepsis, but there is no available immunoassay. Methods: A sandwich ELISA for VS-I was developed, and plasma VS-I was measured in 30 healthy controls and 60 critically ill patients with sepsis. Results: The ELISA showed intra- and inter-Assay coefficients of variations (CVs) below 4 and 9%. Plasma VS-I was significantly increased compared with controls in patients with sepsis, severe sepsis, and sepsis shock (p <0.0001). Receiver operating curve (ROC) analyses indicated that plasma VS-I was more sensitive and specific than plasma CgA to diagnose sepsis and to assess its severity. Conclusions: The measurements of plasma VS-I with this new ELISA may be useful for the clinical investigation of patients with sepsis.

Original languageEnglish
Pages (from-to)430-434
Number of pages5
JournalBiomarkers
Volume17
Issue number5
DOIs
Publication statusPublished - Aug 2012

Fingerprint

Chromogranin A
Immunoassay
Sepsis
Peptides
Plasmas
Enzyme-Linked Immunosorbent Assay
Assays
Biomarkers
vasostatin I
Critical Illness
Shock

Keywords

  • Biomarker
  • Chromogranin A
  • Sepsis
  • Vasostatin

ASJC Scopus subject areas

  • Biochemistry
  • Clinical Biochemistry
  • Health, Toxicology and Mutagenesis

Cite this

Development of an immunoassay for the derived-peptide of chromogranin A, Vasostatin-I (1-76) : Assessment of severity in patients with sepsis. / Chung, Hélène; Corti, Angelo; Crippa, Luca; Schneider, Francis; Metz-Boutigue, Marie Hélène; Garnero, Patrick.

In: Biomarkers, Vol. 17, No. 5, 08.2012, p. 430-434.

Research output: Contribution to journalArticle

Chung, Hélène ; Corti, Angelo ; Crippa, Luca ; Schneider, Francis ; Metz-Boutigue, Marie Hélène ; Garnero, Patrick. / Development of an immunoassay for the derived-peptide of chromogranin A, Vasostatin-I (1-76) : Assessment of severity in patients with sepsis. In: Biomarkers. 2012 ; Vol. 17, No. 5. pp. 430-434.
@article{d48778557d064207b948b9e6cb617940,
title = "Development of an immunoassay for the derived-peptide of chromogranin A, Vasostatin-I (1-76): Assessment of severity in patients with sepsis",
abstract = "Context: Proteolytic fragments of chromogranin A (CgA) including the CgA 1-76 fragment (called vasostatin-I [VS-I]) could be a useful biomarker of sepsis, but there is no available immunoassay. Methods: A sandwich ELISA for VS-I was developed, and plasma VS-I was measured in 30 healthy controls and 60 critically ill patients with sepsis. Results: The ELISA showed intra- and inter-Assay coefficients of variations (CVs) below 4 and 9{\%}. Plasma VS-I was significantly increased compared with controls in patients with sepsis, severe sepsis, and sepsis shock (p <0.0001). Receiver operating curve (ROC) analyses indicated that plasma VS-I was more sensitive and specific than plasma CgA to diagnose sepsis and to assess its severity. Conclusions: The measurements of plasma VS-I with this new ELISA may be useful for the clinical investigation of patients with sepsis.",
keywords = "Biomarker, Chromogranin A, Sepsis, Vasostatin",
author = "H{\'e}l{\`e}ne Chung and Angelo Corti and Luca Crippa and Francis Schneider and Metz-Boutigue, {Marie H{\'e}l{\`e}ne} and Patrick Garnero",
year = "2012",
month = "8",
doi = "10.3109/1354750X.2012.680610",
language = "English",
volume = "17",
pages = "430--434",
journal = "Biomarkers",
issn = "1354-750X",
publisher = "Informa Healthcare",
number = "5",

}

TY - JOUR

T1 - Development of an immunoassay for the derived-peptide of chromogranin A, Vasostatin-I (1-76)

T2 - Assessment of severity in patients with sepsis

AU - Chung, Hélène

AU - Corti, Angelo

AU - Crippa, Luca

AU - Schneider, Francis

AU - Metz-Boutigue, Marie Hélène

AU - Garnero, Patrick

PY - 2012/8

Y1 - 2012/8

N2 - Context: Proteolytic fragments of chromogranin A (CgA) including the CgA 1-76 fragment (called vasostatin-I [VS-I]) could be a useful biomarker of sepsis, but there is no available immunoassay. Methods: A sandwich ELISA for VS-I was developed, and plasma VS-I was measured in 30 healthy controls and 60 critically ill patients with sepsis. Results: The ELISA showed intra- and inter-Assay coefficients of variations (CVs) below 4 and 9%. Plasma VS-I was significantly increased compared with controls in patients with sepsis, severe sepsis, and sepsis shock (p <0.0001). Receiver operating curve (ROC) analyses indicated that plasma VS-I was more sensitive and specific than plasma CgA to diagnose sepsis and to assess its severity. Conclusions: The measurements of plasma VS-I with this new ELISA may be useful for the clinical investigation of patients with sepsis.

AB - Context: Proteolytic fragments of chromogranin A (CgA) including the CgA 1-76 fragment (called vasostatin-I [VS-I]) could be a useful biomarker of sepsis, but there is no available immunoassay. Methods: A sandwich ELISA for VS-I was developed, and plasma VS-I was measured in 30 healthy controls and 60 critically ill patients with sepsis. Results: The ELISA showed intra- and inter-Assay coefficients of variations (CVs) below 4 and 9%. Plasma VS-I was significantly increased compared with controls in patients with sepsis, severe sepsis, and sepsis shock (p <0.0001). Receiver operating curve (ROC) analyses indicated that plasma VS-I was more sensitive and specific than plasma CgA to diagnose sepsis and to assess its severity. Conclusions: The measurements of plasma VS-I with this new ELISA may be useful for the clinical investigation of patients with sepsis.

KW - Biomarker

KW - Chromogranin A

KW - Sepsis

KW - Vasostatin

UR - http://www.scopus.com/inward/record.url?scp=84864607569&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=84864607569&partnerID=8YFLogxK

U2 - 10.3109/1354750X.2012.680610

DO - 10.3109/1354750X.2012.680610

M3 - Article

VL - 17

SP - 430

EP - 434

JO - Biomarkers

JF - Biomarkers

SN - 1354-750X

IS - 5

ER -