Development of bioengineered human larynx

Silvia Baiguera, Alessandro Gonfiotti, Massimo Jaus, Camilla E. Comin, Milena Paglierani, Costantino Del Gaudio, Alessandra Bianco, Domenico Ribatti, Paolo Macchiarini

Research output: Contribution to journalArticle

Abstract

To date, only two human laryngeal allotransplants have been reported and, although they were successful, both patients required life-long immunosuppression. A bioengineered human larynx could represent a possible alternative to allotransplantation. Human larynxes were decellularized enzymatically to obtain acellular matrices. Histological and molecular analysis demonstrated that all cellular components and nuclear material were removed. SEM showed that decellularized matrices retained the hierarchical structures of the native larynx, and mechanical tests demonstrated that the decellularization did not significantly impaired the biomechanically properties of the obtained matrices. Immunohistochemical staining found residual angiogenic factors after decellularization, and CAM analysis demonstrated that acellular laryngeal scaffolds induce a strong in vivo angiogenic response. Using a decellularization method, we are now able to obtain, in a short and clinically useful time, natural bioengineered laryngeal scaffolds which could be use for partial or total implantation in humans.

Original languageEnglish
Pages (from-to)4433-4442
Number of pages10
JournalBiomaterials
Volume32
Issue number19
DOIs
Publication statusPublished - Jul 2011

Keywords

  • Acellular matrix
  • Angiogenesis
  • Decellularization protocol
  • Human larynx
  • Mechanical properties
  • Morphological properties

ASJC Scopus subject areas

  • Biomaterials
  • Bioengineering
  • Ceramics and Composites
  • Mechanics of Materials
  • Biophysics

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  • Cite this

    Baiguera, S., Gonfiotti, A., Jaus, M., Comin, C. E., Paglierani, M., Del Gaudio, C., Bianco, A., Ribatti, D., & Macchiarini, P. (2011). Development of bioengineered human larynx. Biomaterials, 32(19), 4433-4442. https://doi.org/10.1016/j.biomaterials.2011.02.055