Development of ISFET array-based microsystems for bioelectrochemical measurements of cell populations

Sergio Martinoia, Nicola Rosso, Massimo Grattarola, Leandro Lorenzelli, Benno Margesin, Mario Zen

Research output: Contribution to journalArticle

Abstract

Monitoring the bioelectrochemical activity of living cells with sensor array-based microsystems represents an emerging technique in a large area of biomedical applications, ranging from basic research to various fields of pharmacological analyses. The main appeal is the ability of these miniaturised microsystems to perform, in real time, non-invasive in-vitro investigations of the physiological state of a cell population. In this paper, we present two different microsystems designed for multisite monitoring of the physiological state of a cell population. The first microsystem, intended for cellular metabolism monitoring, consists of an array of 12 spatially distributed ISFETs to detect small pH variations induced by the cell population. The second microsystem consists of an array of 40 ISFETs and 20 gold microelectrodes and it has been designed to monitor the electrical activity of neurons. This is achieved by direct coupling of the neuronal culture with the ISFET sensitive layer and by utilising gold microelectrodes for neuronal electrical stimulation.

Original languageEnglish
Pages (from-to)1043-1050
Number of pages8
JournalBiosensors and Bioelectronics
Volume16
Issue number9-12
DOIs
Publication statusPublished - 2001

Fingerprint

Ion sensitive field effect transistors
Microsystems
Cells
Microelectrodes
Gold
Population
Monitoring
Physiologic Monitoring
Electric Stimulation
Sensor arrays
Metabolism
Neurons
Pharmacology
Research

Keywords

  • Bioelectronics
  • Cell-metabolism monitoring
  • Electrophysiological measurements
  • Microsensor array

ASJC Scopus subject areas

  • Biotechnology
  • Analytical Chemistry
  • Electrochemistry

Cite this

Development of ISFET array-based microsystems for bioelectrochemical measurements of cell populations. / Martinoia, Sergio; Rosso, Nicola; Grattarola, Massimo; Lorenzelli, Leandro; Margesin, Benno; Zen, Mario.

In: Biosensors and Bioelectronics, Vol. 16, No. 9-12, 2001, p. 1043-1050.

Research output: Contribution to journalArticle

Martinoia, S, Rosso, N, Grattarola, M, Lorenzelli, L, Margesin, B & Zen, M 2001, 'Development of ISFET array-based microsystems for bioelectrochemical measurements of cell populations', Biosensors and Bioelectronics, vol. 16, no. 9-12, pp. 1043-1050. https://doi.org/10.1016/S0956-5663(01)00202-0
Martinoia, Sergio ; Rosso, Nicola ; Grattarola, Massimo ; Lorenzelli, Leandro ; Margesin, Benno ; Zen, Mario. / Development of ISFET array-based microsystems for bioelectrochemical measurements of cell populations. In: Biosensors and Bioelectronics. 2001 ; Vol. 16, No. 9-12. pp. 1043-1050.
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