Development of mammary and cutaneous gland tumors in transgenic mice carrying the RET/PTC1 oncogene

Giuseppe Portella, Domenico Salvatore, Gerardo Botti, Aniello Cerrato, Li Zhang, Alba Mineo, Gennaro Chiappetta, Giovanni Santelli, Laura Pozzi, Giancarlo Vecchio, Alfredo Fusco, Massimo Santoro

Research output: Contribution to journalArticle

Abstract

RET/PTC1 is a chimeric oncogene created by the fusion of the tyrosine kinase domain of RET to the 5'-terminal region of another gene named H4, So far, this oncogene has been found activated only in human papillary thyroid carcinomas. In order to investigate its transforming properties in vivo, we have produced transgenic mice carrying RET/PTC1 under the control of the H4 promoter. The transgene was expressed in several tissues, consistently with the ubiquitous expression of the wild type H4 gene. Mammary adenocarcinomas and, less frequently, hyperplasia of sebaceous glands and rare benign skin tumors, named pilomatrixomas, developed in these mice. The tumors were shown to express the transgene both at the RNA and protein level. These results demonstrate that the transforming ability of the RET/PTC1 oncogene is not restricted to the thyroid epithelium in vivo. Despite its ubiquitous expression, however, RET/PTC1 was able to induce only a limited number of tumor types; specifically mammary epithelium was affected by transgene expression, thus suggesting that RET/PTC1 is able to couple with transforming pathways specific for these glandular cells.

Original languageEnglish
Pages (from-to)2021-2026
Number of pages6
JournalOncogene
Volume13
Issue number9
Publication statusPublished - 1996

Keywords

  • Mammary
  • Oncogene
  • RET
  • Thyroid
  • Transgenic

ASJC Scopus subject areas

  • Molecular Biology
  • Cancer Research
  • Genetics

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  • Cite this

    Portella, G., Salvatore, D., Botti, G., Cerrato, A., Zhang, L., Mineo, A., Chiappetta, G., Santelli, G., Pozzi, L., Vecchio, G., Fusco, A., & Santoro, M. (1996). Development of mammary and cutaneous gland tumors in transgenic mice carrying the RET/PTC1 oncogene. Oncogene, 13(9), 2021-2026.