Development of microcantilever-based biosensor array to detect Angiopoietin-1, a marker of tumor angiogenesis

Carlo Ricciardi, Sonia Fiorilli, Stefano Bianco, Giancarlo Canavese, Riccardo Castagna, Ivan Ferrante, Gabriella Digregorio, Simone Luigi Marasso, Lucia Napione, Federico Bussolino

Research output: Contribution to journalArticle

Abstract

Microcantilever biosensors have been proposed in the last years as very sensitive mass detectors, but few works focused on the precision and specificity of such tools. We measured the repeatability and reproducibility of our cantilever-based system, proponing the combination of results coming from both the first and second mode of vibration. Then, we optimized two biodesigns (a receptor-based and an antibody-based) to the detection of Angiopoietin-1, a possible marker in tumor progression. The reported results show that our microcantilever-based system can detect Angiopoietin-1 masses of the order of few hundreds of picograms with less than 0.5% of relative uncertainty. We showed that the evaluation of the protein surface density (number of molecules per cm2) could reveal interesting features concerning the multimerization state of the targeted protein. We also performed negative controls (dipping the sample in PBS without proteins) and specificity tests (dipping the sample in PBS with a "false" antigen). The related frequency shifts coming from non-specific interactions were found to be at least one order of magnitude lower than typical variations due to specific protein binding. Thanks to its fine precision and optimal specificity, our microcantilever-based system can be successfully applied as a quantitative tool for systems biology studies such as the comprehension of angiogenic machinery and cancer progression.

Original languageEnglish
Pages (from-to)1193-1198
Number of pages6
JournalBiosensors and Bioelectronics
Volume25
Issue number5
DOIs
Publication statusPublished - Jan 15 2010

Keywords

  • Angiogenesis
  • Microcantilever
  • Precision
  • Protein detection
  • Specificity

ASJC Scopus subject areas

  • Biophysics
  • Biomedical Engineering
  • Biotechnology
  • Electrochemistry

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  • Cite this

    Ricciardi, C., Fiorilli, S., Bianco, S., Canavese, G., Castagna, R., Ferrante, I., Digregorio, G., Marasso, S. L., Napione, L., & Bussolino, F. (2010). Development of microcantilever-based biosensor array to detect Angiopoietin-1, a marker of tumor angiogenesis. Biosensors and Bioelectronics, 25(5), 1193-1198. https://doi.org/10.1016/j.bios.2009.10.006